骨髓DNA甲基转移酶基因在急性髓系白血病患者的表达及意义

Expression of DNMT Gene in Bone Marrow of Patients with Acute Myelogenous Leukemia and Its Significance

本研究旨在探讨DNMT1基因在急性髓系白血病(AML)患者骨髓中的表达及其意义。应用实时定量PCR方法检测30名正常人和126例AML患者骨髓中DNMT1基因的表达。结果表明,30例正常人骨髓中检测到较低水平的DNMT1基因表达;DNMT1基因在AML患者骨髓中的表达水平增高,AML缓解后DNMT1基因的表达水平较初诊时明显降低;DNMT1基因表达水平与AML患者的年龄、性别及初诊白细胞数等临床特征无关;治疗后DN-MT1基因高表达组完全缓解率高于低表达组。结论:骨髓DNMT1基因表达水平在AML发病中可能起重要作用,可作为判断AML预后的指标之一。

This study was aimed to explore the expression and significance of DNMT1 gene in bone marrow of patients with acute myelogenous leukemia（AML）.The expression of DNMT1 gene was detected by real-time PCR in 30 healthy people and 126 AML patients.The results showed that the expression level of DNMT1 gene was lower in the 30 healthy people and was higher in AML patients.There was a marked decline in the expression level of DNMT1 gene after complete remission（CR） as compared with the initial treatment.The expression level of DNMT1 gene did not correlated with age,sex and the clinical characteristics at initial diagnosis such as white blood cell count and chromosomal karyotype in AML patients.The CR rate in AML patients with low expression level of DNMT1 gene was lower than that in those with high expression level.It is concluded that bone marrow DNMT1 gene level may play an important role in AML pathogenesis and can serve as an index in evaluating AML prognosis.