黄芩提取化合物单体SBX抗白血病效应及机制研究

Anti-Leukemic Effect of Skutellaria Extract SBX and Its Mechanisms

本研究探讨黄芩提取化合物单体成分SBX对不同人白血病细胞株的抗白血病效应及其可能的相关机制。体外培养人HL-60、NB4、U937、K562、Jurkat细胞,应用CellTiter-Glo发光法进行细胞活力检测,筛选出敏感细胞株,应用流式细胞术检测SBX对敏感细胞细胞周期的影响,应用蛋白Pathway Array技术检测SBX对敏感细胞蛋白表达的影响。结果显示,SBX(10-200μmol/L,72 h)对各种类型的人白血病细胞均有抑制作用,并呈浓度依赖性(r值分别为0.86,0.88,0.95,0.94,0.96),对HL-60细胞抑制作用最强。流式细胞术显示SBX(50,100μmol/L,48h)对HL-60细胞周期的影响主要是引起G0/G1期阻滞。蛋白Pathway Array技术分析SBX(50μmol/L,48 h)对HL-60蛋白表达的影响显示,p-PKCα/βⅡ、p-p38、Cdc25B、XIAP蛋白表达水平上调,而p-AKT、p-SAPK/JNK、cyclin E、Notch4、Cdk4、Cdc2、Akt、Bcl-2、Bax、cdc42、TNF-α、p27、CaMKKa蛋白表达水平下调。结论:SBX能够抑制各种类型人白血病细胞的增殖,其中敏感细胞株为HL-60。SBX主要影响HL-60的EGFR、Ras/Raf/MAPK和Notch信号传导通路,并通过这些信号传导通路影响细胞周期相关蛋白表达的水平,导致细胞周期G0/G1期阻滞。

This study was aimed to explore the anti-leukemic effect of skutellaria extract SBX in human leukemia cell lines and its mechanism.The leukemia cell lines,including HL-60,NB4,U937,K562 and Jurkat,were cultured in vitro and proliferative inhibition of these cell lines was detected by CellTiter-Glo Luminescent Cell Viability Assay in order to screene the most sensitive cell line.The effect of SBX on cell cycle was analyzed by flow cytometry and the protein expressions determined by Protein Pathway Array respectively.The results indicated that SBX（10-200 μmol/L,for 72 h） significantly inhibited the proliferation of different leukemia cell lines in a dose-dependent manner（r value was 0.86,0.88,0.95,0.94,0.96,respectively）,the HL-60 was the most sensitive cell line.Flow cytometric analysis showed that SBX（50,10 μmol/L,for 48 h） arrested HL-60 cells in the G0/G1 phase.In addition,protein expression of p-PKC α/βⅡ,p-p38,Cdc25B,XIAP of HL-60 cells increased,and p-AKT,p-SAPK/JNK,Notch4,Cdk4,Cdc2,cyclin E,Akt,Bcl-2,Bax,cdc42,TNF-α,p27,CaMKKa decreased after exposure to SBX（50 μmol/L,for 48 h）.It is concluded that SBX can inhibit the proliferation of different leukemia cell lines,and HL-60 is a sensitive cell line.SBX significantly influences EGFR,Ras/Raf/MAPK and Notch signaling pathway,through which effects the expression of cell cycle-related proteins resulting in arrest of HL-60 cells in G0/G1.