期刊文献+

干扰素γ对人脐带间充质基质细胞黏附分子表达的影响

Effects of Interferon-γ on Expression of Adhesion Molecules in Human Umbilical Cord Mesenchymal Stromal Cells
下载PDF
导出
摘要 本研究观察干扰素γ(IFN-γ)对体外培养人脐带间充质基质细胞(UC-MSC)表面黏附分子表达水平的影响。采用组织块移行法培养人UC-MSC,并进行细胞表面抗原、成骨和成脂鉴定。向第3代UC-MSC加入不同浓度的IFN-γ,干预24 h后收集细胞,应用流式细胞仪检测CD54、CD58、CD62p、CD62L、CD44、CD49d、CD102及CD106黏附分子的表达水平。结果表明,生理状态下,CD106、CD62P、CD62L和CD102阳性表达率极低(均<1%),CD54表达最高(41.58±0.83)%;经IFN-γ干预后,CD102、CD106、CD62L、CD62p阳性表达率略有升高,但总体变化不明显(均<5%);CD54、CD58阳性表达率与IFN-γ呈浓度依赖性,最高达(59.66±1.36)%,(43.96±0.62)%;CD49d的阳性表达率在100 U/ml时达到峰值(51.33±0.74)%,CD44在浓度为1 000 U/ml时阳性表达率最高(73.22±1.93)%。结论:IFN-γ可显著提高UC-MSC表面CD54、CD58、CD44、CD49d的阳性表达率,但对CD102、CD106、CD62P和CD62L作用不明显。 This study was purposed to investigate the effects of interferon(IFN)-γ on expression of adhesion molecules in mesenchymal stromal cells derived from human umbilical cord tissue(UC-MSC).The UC-MSC were isolated from human umbilical cord by tissue culture.The expressions of specific markers on UC-MSC were detected by flow cytometry in the physiological condition.The adipogenic and osteogenic induction of UC-MSC was detected by alizarin and Oil red O staining.UC-MSC were exposed to IFN-γ(100,1 000,10 000 U/ml) for 24 h,the expressions of CD54,CD58,CD44,CD49d,CD62p,CD62L,CD102 and CD106 on cell surface were detected using flow cytometry.The results showed that in physiological condition,UC-MSC extremely low expressed CD102,CD106,CD62P,CD62L,while the expression of CD54 was relatively high(41.58±0.83)%.When stimulated by IFN-γ,the expression of CD102,CD106,CD62P,CD62L increased slightly,but still low(5%),while CD54 and CD58 upregulated concentration-dependently up to(59.66±1.36)% and(43.96±0.62)% respectively.The expression of CD49d upregulated to(51.33±0.74)% when UC-MSC exposed to IFN-γ 100 U/ml.CD44 increased to(73.22±1.93)% when UC-MSC exposed to IFN-γ 1 000 U/ml.It is concluded that IFN-γ can elevate significantly the expression of CD54,CD49d,CD44 and CD58,but has no significant effect on CD102,CD106,CD62P and CD62L expression on the surface of UC-MSC.
出处 《中国实验血液学杂志》 CAS CSCD 北大核心 2012年第5期1195-1199,共5页 Journal of Experimental Hematology
基金 甘肃省科技重大专项(编号1102FKDA005)
关键词 脐带间充质基质细胞 黏附分子 干扰素Γ human umbilical cord mesenchymal stromal cells adhesion molecule interferon
  • 引文网络
  • 相关文献

参考文献14

  • 1马春朋,王庆胜,刘晓丽.人类脐带Wharton’sjeny来源的基质细胞.国际生物医学工程杂志,2008;31(5):305-307.
  • 2Lu LL, Liu YJ, Yang SG, et al. Isolation and characterization of human umbilical cord mesenchymal stem cells with hematopoiesis- supportive function and other potentials. Haematologica, 2006; 91 (8) : 1017 -1026.
  • 3Najar M, Raicevic G, Boufker H, et al. Modulated expression of adhesion molecules and galectin-l: role during mesenchymal stromal cell immunoregulatory functions. Exp Hematol, 2010; 38 (10) :922 -932.
  • 4Segers VF, Riet IV, Andries LJ, et al. Mesenchymal stem cell adhesion to cardiac microvascular endothelium: activators and mechanisms. Am J Physiol Heart Circ Physiol, 2006; 290 (4): 1370 - 1377.
  • 5贾爱军,马存根,梁丽云,张利宏,赵海宝.脑缺血大鼠干扰素γ与细胞间黏附分子-1表达相关性研究[J].中国脑血管病杂志,2005,2(4):169-172. 被引量:2
  • 6Jaruga B, Hong F, Kim WH, et al. IFN-gamma/STAT1 acts as a proinflammatory signal in T cell-mediated hepatitis via induction of multiple chemokines and adhesion molecules: a critical role of IRE-1. Am J Physiol Gastrointest Liver Physiol, 2004 ; 287 ( 5 ) : 1044 - 1052.
  • 7时青云,刘淑芸,彭芝兰,赵霞.干扰素、地塞米松对妊娠肝内胆汁淤积症患者脐带内皮细胞粘附分子-1表达的影响[J].中华妇产科杂志,2001,36(10):630-631. 被引量:4
  • 8Ruster B, Gottig S, Ludwig RJ, et al. Mesenchymal stem cells display coordinated rolling and adhesion behavior on endothelial cells. Blood, 2006 ; 108 ( 12 ) : 3938 - 3944.
  • 9Zhang J, Alcaide P, Liu L, et al. Regulation of endothelial cell adhesion molecule expression by mast cells, macrophages, and neutrophils. PLoS One ,6 ( 1 ) : 1 - 10.
  • 10张胜娟,闫志鹏,宋秀君,张晓融,卞小芸.体外培养人角膜上皮细胞中CD44的表达及IFN-γ和雷公藤多甙对其的影响[J].眼科研究,2009,27(3):188-191. 被引量:1

二级参考文献26

共引文献5

;
使用帮助 返回顶部