摘要
Micro-RNAs（miRNAs） have been found to be implicated in a very wide range of physiological processes.This study was aimed to investigate the regulation of miRNA-429（miR-429） in gastric cancer cells on cell proliferation and apoptosis.Quantitative PCR was employed to detect the expressions of miR-429 after eukaryotic expression plasmid of miR-429 and its inhibitor were transiently transfected into poorly differentiated human gastric can-cer cell line BGC823.The 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） reduction as-says were used to examine proliferation ability.Apoptosis was analyzed by flow cytometry after transfection.The results showed that 48 h after transfection,overexpression of miR-429 reached maximum efficiency.Compared with mock transfection,miR-429 inhibited tumor cell proliferation significantly（P 〈 0.05） at 48 h and 72 h.of Overexpression of miR-429 promoted tumor cell apoptosis when compared with mock transfected cells（P 〈 0.05）.On the contrary,miR-429 inhibitor promoted tumor cell proliferation and inhibited apoptosis when compared with controls（P 〈 0.05）.Our results suggested that miRNA-429 may serve as a tumor suppressor during tumorigenesis of gastric cancer and may be a potential gastric cancer therapeutic target.
Micro-RNAs（miRNAs） have been found to be implicated in a very wide range of physiological processes.This study was aimed to investigate the regulation of miRNA-429（miR-429） in gastric cancer cells on cell proliferation and apoptosis.Quantitative PCR was employed to detect the expressions of miR-429 after eukaryotic expression plasmid of miR-429 and its inhibitor were transiently transfected into poorly differentiated human gastric can-cer cell line BGC823.The 3-（4,5-dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） reduction as-says were used to examine proliferation ability.Apoptosis was analyzed by flow cytometry after transfection.The results showed that 48 h after transfection,overexpression of miR-429 reached maximum efficiency.Compared with mock transfection,miR-429 inhibited tumor cell proliferation significantly（P 〈 0.05） at 48 h and 72 h.of Overexpression of miR-429 promoted tumor cell apoptosis when compared with mock transfected cells（P 〈 0.05）.On the contrary,miR-429 inhibitor promoted tumor cell proliferation and inhibited apoptosis when compared with controls（P 〈 0.05）.Our results suggested that miRNA-429 may serve as a tumor suppressor during tumorigenesis of gastric cancer and may be a potential gastric cancer therapeutic target.
基金
supported by the National Natural Science Foundation of China(No.30973489)
