早期膝骨关节炎患者血清和关节液中COMP含量测定及其临床意义

Clinical Significance of the COMP Content Measurement in Serum and Joint Fluid of Early Osteoarthritis Patients

目的：通过检测早期膝关节骨关节炎（osteoarthritis，OA）患者血清和关节液中软骨寡聚基质蛋白（cartilage oligomeric matrix protein，COMP）含量并对比正常人血清COMP含量来分析其临床意义。方法：选取2010年11月-2011年11月间在我院门诊因早期膝关节OA首次行关节腔玻璃酸钠注射患者37例，其中男21例，女16例，平均年龄（61．70±4．31）岁，留取其关节液和血液样本；另取同期在门诊体检的健康人37例，其中男12例，女25例，平均年龄（60．59±3．12）岁，留取其血液标本。标本离心后取上清液，集中采用ELISA法检测其COMP含量并进行统计分析。结果：37例早期膝关节OA患者血清中COMP含量（5．84±0．74）ng／ml，关节液中COMP含量（6．37±0．73）ns／ml，两者比较差异有统计学意义（P〈0．05）。37例健康对照组血清中COMP含量（4．08±0．41）ng／ml，与早期膝关节OA患者血清中COMP含量相比，差异有统计学意义（P〈0．05）。结论：早期膝关节OA患者，其关节液或血清中COMP含量较正常人有所增高，提示COMP可以作为OA早期诊断的一项辅助指标。

Objective：To detect the COMP content measurement in serum and joint fluid of early osteoarthritis patients and analyze its meaning compared with normal people. Methods：From Nov. 2010 to Nov. 2011, 37 early osteoarthritis patients who were treated by injecting sodium hyaluronate into the knee joint for the first time were selected（21 males and 16 females, averaged 61.70 ±4.31 years） , their blood and knee joint fluid were drawn. The comparison was done between them and 37 normal people（ 12 males and 25 females, averaged 60.59 ± 3.12 years）, their blood was kept when they had physical examination. The content measurement of COMP in these samples was detected with ELISA. Results：The average COMP content measurement of 37 patients was （ 5.84 ± 0.74） ng,/ml in the serum and （6.37 ± 0. 73 ）ng/ml in the joint fluid. The result of control group was （4.08 ± 0.41 ）ng/ml. There was statistical significance between their difference. Conclusions：The measurement of COMP content in serum and joint fluid of early osteoarthfitis patients is higher than normal people ,which shows that COMP can be an indicator in diagnosis of early osteoarthfitis.