甘蓝Ri T-DNA转化根的植株再生

PLANT REGENERATION OF Ri T-DNA TRANSFORMED ROOTS OF CABBAGE

利用发根农杆菌(Agrobacterium rhizogenes)的遗传转化作用,从甘蓝下胚轴切段上诱发产生了Ri T-DNA转化根。在无激素MS琼脂培养基上,经过长达4个月的连续培养后,有些转化根的根段组织脱分化形成愈伤组织块,然后又再分化形成茎芽。在含有激动素的MS琼脂培养基上,转化根不经过愈伤组织阶段直接分化出茎芽,其中以14经μmol/L激动素处理的生茎效果最佳。在MS液体培养基内加入4.5βmol/L BA也增加了转化根上的茎芽数。所有这些茎芽能够进一步生长,并且在生根培养基上生根,长成小植株。

Ri T-DNA transformed roots were induced on hypocotyl segments of cabbage with Agrobacterium rhizogenes. On solid MS medium without hormones, some parts of a few of transformed roots were dedifferentiated into callus clump, and then redifferentiated into shoot buds since the roots had been cultured for 4 months. On solid MS medium supplemented with kinetin, shoot buds were differentiated directly along transformed roots without callus formation. Among several concentrations, 14 μmol/L kinetin gave rise to the best results for differentiation of shoot buds. 4.5μmol/L at a concentration of BA in liquid MS medium also increased shoot buds from transformed roots.All of these shoot buds grew further, and were rooted on root induction medium, developling into young plants.