类质粒S-1与玉米和高粱基因组同源性的研究

STUDY ON THE HOMOLOGY BETWEEN PLASMIDLIKE S-1 DNA AND THE GENOMES OF MAIZE AND SORGHUM

在S型和N型玉米线粒体基因组中,存在S-1、S-2类质粒的同源序列。研究表明,在正常细胞质的线粒体DNA(mtDNA)主环(570kb)上,与S-1同源的这段序列位于6.9kb的BamH Ⅰ片段之中。此外,在N型玉米核基因组中,也存在与类质粒S-1的同源序列。

The results of hybridization of plasmid-like S-1 DNA from mtDNA in Stype cytoplasmic maize to Southern blot of BamH1 digested mtDNA and nDNA from four normal cy toplasmic maize inbreds (Chao23, D69, C3, K64)show that therc are homology between S-1 and mtDNA and homology between S-1 and nDNA. When the probe is changed into cloned S-1(Two ends lack 128bp), the results of hybridization to Southern blot of Barn H I digested mtDNA from normal cytoplasmic maize inbred Huo Bai and sorghum hybrid Xin 7 indicate that there is homology between S-1 and mtDNA from maize and no homology between S-1 and mtDNA from sorghum. These data may be helpful in using S-1 as vector or in using S-1 to construct vectors so as to promole foreign genes to be transfered into monocotyledon.