一种厌氧微观定量研究新方法

A Novel Quantitative Approach to Study Dynamic Anaerobic Process at Micro Scale

厌氧消化由于低成本和能源回收等优点越来越引起广泛关注.为克服现有方法研究动态厌氧过程的不足,基于微反应器和定量图像分析技术开发出一种新型的污泥层面的微观定量方法.试验首次展示了静态下颗粒污泥产气的动态过程和特点.试验结果重复性好.静态产气可分为3个阶段,分别是高速线性增加阶段、减速增加阶段和低速线性增加阶段.初始有机负荷较高时,高速线性增加阶段比较长,产气速率也比较高.结果表明,微反应器中进行厌氧产甲烷过程是可行的,该方法可靠,能够在微观层面直观地展示厌氧反应的动态过程,研究结果有利于增进对厌氧过程理解.

Anaerobic digestion is attracting more and more interests because of its advantages such as low cost and recovery of clean energy etc.In order to overcome the drawbacks of the existed methods to study the dynamic anaerobic process,a novel microscopical quantitative approach at the granule level was developed combining both the microdevice and the quantitative image analysis techniques.This experiment displayed the process and characteristics of the gas production at static state for the first time and the results indicated that the method was of satisfactory repeatability.The gas production process at static state could be divided into three stages including rapid linear increasing stage,decelerated increasing stage and slow linear increasing stage.The rapid linear increasing stage was long and the biogas rate was high under high initial organic loading rate.The results showed that it was feasible to make the anaerobic process to be carried out in the microdevice;furthermore this novel method was reliable and could clearly display the dynamic process of the anaerobic reaction at the micro scale.The results are helpful to understand the anaerobic process.