酶法催化丙酮酸和植物甾醇合成植物甾醇酯

Enzymatic synthesis of phytosterol esters with pyruvate and phytosterol

以脂肪酶Lipozyme 435为催化剂,研究了植物甾醇与丙酮酸在有机溶剂正己烷中酯化合成植物甾醇酯(PE)的工艺,最佳工艺条件为:分子筛添加量40 mg/mL,反应温度55℃,底物摩尔比1∶5(植物甾醇与丙酮酸摩尔比),酶添加量5%(以丙酮酸质量计),反应时间20 h。在最优反应条件下,酯化率为93.02%。研究表明植物甾醇和丙酮酸可以酯化合成植物甾醇酯,且可得到较高的酯化率。

The lipase Lipozyme 435 was used as catalyst, and phytosterol esters （PE） were synthesized in n - hexane by esterification of phytosterol and pyruvate. The optimal synthesis conditions were as follows ： molecular sieves amount 40 mg/mL, reaction temperature 55 ℃, molar ratio of phytosterol to pyruvate 1 ： 5, enzyme dosage 5 % （ based on the mass of pyruvate）, reaction time 20 h. Under the optimal condi- tions, the esterification rate was 93.02%. The results showed that the synthesis of phytosterol esters with pyruvate and phytosterol was possible, and a higher esterification rate could be obtained.