亚砷酸钠对人支气管上皮细胞的氧化损伤作用

Oxidative damage induced by sodium arsenite in human bronchial epithelial cells in cultured

目的探讨不同浓度亚砷酸钠对永生化人支气管上皮细胞氧化损伤的影响。方法体外培养永生化人支气管上皮(HBE)细胞,加入终浓度为0～50 000μmol/L的亚砷酸钠溶液暴露24 h,测定细胞活性;加入终浓度为0～6μmol/L的亚砷酸钠溶液暴露24 h,测定活性氧(ROS)、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力及细胞DNA链断裂情况。结果与对照组比较,各浓度亚砷酸钠染毒组HBE细胞内ROS、MDA含量和Olive尾矩均显著升高,细胞存活率和SOD活力均显著下降,差异有统计学意义(P<0.05);且随着亚砷酸钠染毒浓度的升高,HBE细胞内MDA、ROS含量和Olive尾矩均呈明显的上升趋势(P<0.05),细胞存活率和SOD活力均呈明显的下降趋势(P<0.05)。结论亚砷酸钠可以诱导HBE细胞氧化损伤增加,提示氧化应激可能是亚砷酸钠致HBE细胞毒作用机制之一。

Objective To study the effect of oxidative damage induced by sodium arsenite in human bronchial epithelial cells （HBE cells）. Methods The cultured HBE cells were treated with sodium arsenite at the doses of 0-50 000 μmol/L respectively for 24 h. The cell viability was tested by Cell Counting Kit-8 （CCK-8）. HBE cells were exposed to sodium arsenite at the doses of 0-6 μmol/L respectively for 24 h, and the reactive oxygen species（ROS） level,the malondialdehyde（MDA） and the superoxi dismutase （SOD） content in HBE cells were detected respectively. Single cell gel electrophoresis （SCGE） was applied to the quantitative analysis of DNA damage in HBE cells. Results Compared with the control group, the level of ROS and the content of MDA were significantly increased （P〈0.05）, while the content of SOD was significantly decreased in exposure groups（P〈0.05）. Sodium arsenite could significantly increase DNA Olive tail moment in HBE ceils in exposure groups compared with the control group （P〈0.05）. Conclusion Sodium arsenite may increase the oxidative damage in HBE cells incuhured. It is suggested that oxidative stress might be an important mechanism for the toxicity of sodium arsenite to HBE cells.