高原低氧对大鼠血清中低氧诱导因子-1α含量和血浆中活性氧含量及肝细胞超微结构的影响

Influence of high altitude hypoxia on HIF-1α expression in serum,ROS level in plasma and cell ultrastructure of rats liver

目的探讨高原低氧对大鼠血清中低氧诱导因子-1α(HIF-1α)含量和血浆中活性氧(ROS)含量及肝细胞超微结构的影响。方法将50只健康成年SPF级雄性SD大鼠随机分为对照组(海拔2 262 km)和高原组(海拔4 300km),每组25只。喂养30 d后,测定血清HIF-1α和血浆ROS水平,观察肝细胞超微结构。结果与对照组比较,高原组大鼠血清中HIF-1α含量明显增高,血浆中ROS含量明显降低,差异均有统计学意义(P<0.01);肝细胞线粒体肿胀,粗面内质网扩张,部分轻度脱粒现象。结论高原低氧环境可能通过调节ROS、HIF-1α水平保护细胞,促进低氧适应。

Objective To study the influence of high altitude hypoxia on the levels of HIF-1α, ROS and the hver ceils uhrastructure of rats. Methods A total of 50 healthy adult male SD rats were randomly divided into two groups： control group （altitude： 2 262 km） and plateau group （altitude ： 4 300 km）. After having been feed for 30 days, the samples of blood and liver of rats were collected. The HIF-1α level and ROS level were measured by ELISA approach,and the liver cells ultrastructure were observed under the electron microscope. Results The HIF-1α level in plateau group [（9.64±4.67） ng/ml] were significantly higher than those in control group[（3.47±1.54） ng/ml], the ROS level in plateau group[（120.03±61.86） U/L] were significantly lower than those in control group [（240.63±91.48） U/L]. The swelling of mitochondria and rough endoplasmic reticulum in hypoxia group was observed under the electron microscope. Conclusion It is the possible mechanism for hypoxia adaptation that adjusting the ROS, HIF-1α level to promote cell survival in high altitude hypoxia environment.