摘要
微小按蚊Anophelesminimus是云南省勐腊地区的重要传疟媒介，DDT和拟除虫菊酯类杀虫剂在该地区长期大量使用，研究微小按蚊与kdr抗性密切相关的para型钠通道基因对该地区选择合理的媒介控制方案预防和阻断疟疾的流行提供理依据。本文分别于2010和2011年9～10月在云南省勐腊县龙塘村和东方红村现场采集微小按蚊后，用分子学方法进行分型，根据GenBank发布的微小按蚊para钠通道IIS6段基因组DNA序列设计特异性引物，扩增现场采集的微小按蚊的该段基因，并测序对比。通过对415个个体的检测发现，1014位点编码的TTA氨基酸密码子没有突变，说明截止目前云南勐腊地区微小按蚊对DDT和拟除虫菊酯类杀虫剂抗性的kdr分子机制尚未出现，其击倒抗性较低，对DDT和拟除虫菊酯类杀虫剂仍然保持敏感。
Anopheles minimus is an important vector of malaria in Mengla, Yunnan Province, China. DDT and pyrethroid have been applied in this region for a long time. To provide reasonable basis for vector control programs and blocking the prevalence of malaria, An. minimus were collected from this area and were determined genotyping of para-type sodium channel genes, which is closely related to knockdown resistance. Amplified the sodium channel gene from An. minimus in Mengla, Yunnan Province, were found not mutation at the 1014 loci among 415 individual tests. It is suggested that the knockdown resistance level of An. minimus in Mengla is undetectable, the An. minimus is still sensitive to pyrethroid insecticides
出处
《寄生虫与医学昆虫学报》
CAS
2012年第3期156-160,共5页
Acta Parasitologica et Medica Entomologica Sinica
基金
基金项目：国家自然科学基金项目（No.30970411）
