盆炎汤对感染解脲脲原体小鼠生殖细胞凋亡的影响

The Effects of Pen Yan Tang on Germ Cells Apoptosis of Mice Colonized with Ureaplasma Urealyticum

目的:利用小鼠生殖道感染支原体的模型,研究盆炎汤对小鼠生殖细胞凋亡的影响。方法:昆明雌小鼠63只,随机分7组,采用注射外源性雌激素、阴道接种血清8型Uu菌株的方法建立模型。西药采用阿奇霉素、中药用朱南孙教授的经验方盆炎汤。结果:1)白介素-1β模型组与正常组相比较有统计学意义(P<0.05),其余各组之间相比较无统计学意义(P>0.05);白介素-6各组间相比较无统计学意义(P>0.05);2)子宫组织细胞凋亡率各组之间无统计学意义(P>0.05);阴道组织细胞凋亡各组与模型组相比较均有统计学意义(P<0.05);但各干预组之间无统计学意义(P>0.05)。结论:盆炎汤对Uu的生长有一定的抑制作用;盆炎汤可以减轻支原体对机体的病理损伤,抑制炎症因子的产生及生殖细胞的凋亡。

Objective：Through investigating the expressions of cells apoptosis of vagina and uteri, to discus the mechanism of Pen Yan Tang curing Ureaplasma urealyticum. Methods： Young adult female mice of strain KM were used. Sixty - three mice were divided into 7 groups randomly to be observed and compared in the trial. The medicine were Azithromycin and Pen Yan Tang. All mice were given Es- tradiol benzoate subcutaneously on four occasions, at weekly intervals. The serovar 8 of Ureaplasma urealytieum was inoculated intravagi- nally into all of the mice except the normal group at the same time as the mice received the second dose of Estradiol benzoate. Results ： 1 ）The IL - 113 level of the model group was increased, the difference with normal group was statistically significant （P 〈 0. 05 ） ;The IL - 113 levels of all the treatment groups had no significantly difference （ P 〉 0. 05 ） ; The IL - 6 levels of all the groups had no significantly difference （P 〉 O. 05 ） ; 2 ）The uteri cells apoptosis rates of all the groups had no significantly difference （P 〉 0. 05 ） ;The vagina ceils ap- optosis rate of the model group was higher than the other groups（P 〈0. 05） ;the vagina cells apoptosis rates of all the treatment groups had no significantly difference （ P 〉 0. 05 ）. Conclusion： Pen Yan Tang can inhibit the growth of Uu. Pen Yan Tang could reduce the path- ological damage to the host,inhibit the expressions of inflammatory factor and germ cell apoptosis.