白头翁汤激活NLRP3炎症小体治疗炎症性肠病的研究

Pulsatilla Decoction Treat Inflammatory Bowel Disease by Activating NLRP3

目的:探讨白头翁汤治疗炎症性肠病(IBD)的分子机制。方法:84只BALB/c小鼠随机分为7组(n=12):乙醇对照组、模型组、SASP组、白头翁汤组(低、中和高剂量组)和zVAD组。各组分别灌胃和腹腔给药。疗程结束后取小鼠结肠组织分别利用荧光定量RT-PCR、Elisa和免疫组化等方法检测NLRP3、ASC、Caspase-1、IL-18和IL-1β在结肠组织中的表达量。结果:与模型组比较,SASP组和中、高剂量白头翁汤对IBD有显著的治疗作用(P<0.01),但这种治疗作用在zVAD组被抑制(P<0.05)。同时,中、高剂量白头翁汤能够升高NLRP3、ASC、Caspase-1、IL-18和IL-1β在结肠组织的表达(P<0.05),而zVAD能抑制这种上调(P<0.05)。结论:白头翁汤可能通过激活NLRP3炎症小体进而促进相应的炎症因子包括IL-18及IL-1β的生成来发挥治疗IBD的作用。

Objective：To investigate the molecular mechanisms underlying the treatment of inflammatory bowel disease（IBD） by Pulsatilla decoction.Methods：84 BALB/c mice were randomly divided into ethanol control group,model group,SASP group,Pulsatilla decoction group（further divided into low,middle and high dose group）and zVAD group（n=12）.Intragastric and celiac drug administration were used in each group respectively.The expression of NLRP3,ASC,Caspase-1,IL-18 and IL-1β in the colons were detected by fluorescence quantitative RT-PCR,Elisa and immunohistochemistry after treatment respectively.Results：Compared with the model group,SASP group,middle and high dose group could treat IBD effectively（P0.01）,while this effect was restrained in zVAD group（P0.05）.In the meantime,middle and high dose group could effectively raise the expression of NLRP3,ASC,Caspase-1,IL-18 and IL-1β in the colons（P0.05）,while this effect was also inhibited in zVAD group（P0.05）.Conclusion：Pulsatilla decoction is likely to exert their therapeutic effect by activating NLRP3 inflammasome which further promote the formation of the corresponding inflammation factors such as IL-18 and IL-1β.