孤岛油田中一区馆3区块内源微生物营养体系优选及现场应用

Research and field application of activator formulas to endogenous microorganism,Gudao Zhongyi Ng3 block

为了激活注入水中的内源微生物,对孤岛油田中一区馆3区块聚合物驱后加入的营养体系进行了筛选。结果表明,质量浓度为4g/L的葡萄糖、0.4g/L的蛋白胨、0.2g/L的酵母粉、0.4g/L的硝酸铵和0.2g/L的磷酸氢二铵组成了该区块最佳的营养体系。在65℃和10MPa的高温、高压条件下,该体系可以充分激活注入水中的内源微生物,使菌液密度增至1.48×109个/mL,乙酸质量浓度上升至0.6g/L,且菌液的表面张力降低至33.2mN/m。物理模拟驱油实验结果表明,在油藏环境下应用该营养体系激活内源微生物,原油采收率可提高6.4%。现场试验结果表明,截止到2011年6月30日中一区馆3区块共注入该营养体系405t,累积增油量约为1.0×104t。

The activation of Zhongyi Ng3 of Gudao after polymer flooding by means of different activator formulas is studied herein. Screening results reveal that the optimal composition of the activator formula is as follows (g/L) : glucose 4, peptone 0. 4, yeast extract 0. 2, NH4 NO3 0.4, (NH4) 2PO4 0.2. The detailed results show that, at 65℃ and 10 MPa the endogenous microorganism can be activated evidently to 1.48×109 cfu/mL via the activator formula consisted of glucose and yeast extract powder. Simultaneously, the concentration of acetic acid is increased distinctly about 600 mg/L and the surface tension of water is reduced to 33. 2 mN/m. Physical simulation experiment shows that the endogenous microorganism can enhance the oil recovery by 6. 4 percent with the activator formula. Field test shows that the amount of this activator formula to Zhongyi Ng3 block is 405 t by the end of June 2011, and the cumulative oil yield increases by 1.0×104 t.