转录因子AtWRKY28亚细胞定位及在非生物胁迫下的表达分析

Subcellular Localization and Expression Analysis of Transcription Factor AtWRKY28 under Biotic Stresses

WRKY转录因子在调控植物逆境诱导反应、生长发育及信号转导等方面发挥着重要的分子生物学功能。对转录因子AtWRKY28进行了亚细胞定位,并采用荧光定量PCR技术,对其进行表达分析。结果表明:AtWRKY28在细胞核中行使功能且其表达受多种非生物逆境胁迫因子的影响,其中受机械损伤、高盐的诱导尤为明显,说明该基因可能在响应逆境胁迫中发挥一定的功能。此外,对启动子序列的生物信息学分析发现,AtWRKY28基因的启动子包含多个与非生物逆境反应相关的顺式作用元件。

The transcription factor WRKY family is one type of key regulatory components for plant development and defense against stress factors. In this paper, we found that AtWRKY28 gene was located in the nucleus, and the expression profiles of AtWRKY28 genes under abiotic stress were analyzed by q-PCR. The results showed that the expression of AtWRKY28 changed significantly during stress treatments including wounding, NaC1 and ABA, suggesting a specific role of AtWRKY28 in adaptation to environmental stresses. In addition, we analyzed the sequence of the promoters with bioinformatics approach, some cis-elements involved in abiotic stresses and hormonal responses were revealed. These results provided important information for studying the biological functions of AtWRKY28 gene.