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人卵颗粒细胞凋亡对卵母细胞发育潜能的影响 被引量：4

Effect of apoptosis of granular cells on developmental potential of human oocytes
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摘要 目的:研究人卵母细胞发育潜能与颗粒细胞凋亡之间的关系。方法:将卵子在体外经过28～30 h培养后,取其颗粒细胞,用TUNEL法进行凋亡率的分析,并与卵子的成熟情况和卵子的评分及形成胚胎结果进行对照。结果:培养后,随着卵母细胞成熟度的降低,凋亡率升高;而且随着卵母细胞评分和发育潜能的降低,颗粒细胞凋亡率逐渐升高。结论:卵子发育潜能与颗粒细胞凋亡之间关系密切,通过测定颗粒细胞的凋亡率可预测未成熟卵母细胞的发育潜能。 Objective：To explore the relationship between developmental potential of human oocytes and apoptosis of granular cells. Methods：After culturing oocytes in vitro for 28-30 hours,the granular cells were obtained,TUNEL method was used to analyze the apoptotic rate,then the results were compared with the maturation situation of oocytes,the score of oocytes,and the embryonic outcome. Results：After culture,the apoptotic rate of granular cells increased with the decrease of maturation degree of oocytes,the decrease of oocytes score,and the decrease of developmental potential of oocytes. Conclusion：The developmental potential of oocytes is closely related to apoptosis of granular cells,the developmental potential of immature oocytes can be predicted by measuring the apoptotic rate of granular cells.
作者 曹义娟
机构地区 东南大学医学院附属徐州医院 江苏省徐州市中心医院妇产科
出处 《中国妇幼保健》 CAS 北大核心 2012年第30期4780-4782,共3页 Maternal and Child Health Care of China
关键词 颗粒细胞 细胞凋亡 卵母细胞 体外成熟 Granular cell Apoptosis Oocyte In vitro maturation
分类号 R321.1 [医药卫生—人体解剖和组织胚胎学]
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参考文献7

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同被引文献41

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中国妇幼保健
2012年 第30期

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