白花马蹄莲离体快繁技术研究

Studies on Tissue Culture of Zantedeschia aethiopica

[目的]建立白花马蹄莲的离体快繁体系。[方法]选取白花马蹄莲球茎和叶片作为试验材料,采用组培法进行繁殖试验。[结果]接种前球茎的消毒时间以8 min为宜,叶片的消毒时间以4～6 min为宜,最佳球茎诱导分化培养基为MS+2.0 mg/L BA+0.1 mg/LNAA,最佳叶片诱导分化培养基为MS+1.0 mg/L BA+0.5 mg/L NAA;在相同的温度、湿度和光照条件下,应选择马蹄莲球茎作为培养材料;最佳增殖培养基为MS+1.0 mg/L BA;最佳生根培养基为MS+0.1 mg/L IBA;最佳移栽基质为珍珠岩∶蛭石∶草炭土∶沙子=2∶2∶4∶1;最佳移栽时期为生根培养后10～15 d。[结论]为白花马蹄莲的组织培养提供了参考依据。

[Objective] To establish rapid propagation system for Zantedeschia aethiopica.[Method] Selecting Z.aethiopica corm and leaf as material,the tissue culture method was adopted.[Result] The results showed that disinfect the bulb 8 minutes before inoculation and the leaf 4-6 minutes.The best inductive medium for bulb was MS+2.0 mg/L BA+0.1 mg/L NAA,and the best inductive medium for leaf was MS+1.0 mg/L BA+0.5 mg/L NAA.Under the same conditions of temperature,humidity and illumination,the bulb should be chosed as meterials.The best proliferation medium was MS+1.0 mg/L BA.The best rooted medium was MS+0.1 BA.The best transplanting matrice was perlite∶vermiculite∶turfy soil∶sand=2∶2∶4∶1.The best transplanting time was rooting culture for 10-15 days.[Conclusion] The study will provide reference basis for tissue culture of Z.aethiopica.