东北刺人参愈伤组织诱导和继代的研究

Studies on the Callus Inducement and Subculture of Oplopanax elaus Nakai

[目的]研究东北刺人参愈伤组织的诱导和继代方法。[方法]以取自长白山的刺人参植株为材料,研究不同外植体、培养基、激素对刺人参愈伤组织诱导的影响。[结果]叶片为刺人参组织培养的最适宜外植体;5月为外植体的最佳取材时期;以MS为基本培养基,并添加1.0 mg/L 2,4-D和1.0 mg/L 6-BA的培养基中愈伤组织诱导率最高;最佳继代培养基为MS+6-BA 2.0 mg/L+NAA 0.2mg/L。[结论]东北刺人参的最适愈伤诱导培养基和继代培养基分别是:MS+2,4-D 1.0 mg/L+6-BA 1.0 mg/L和MS+6-BA 2.0 mg/L+NAA 0.2 mg/L。

[Objective] To study the callus induction and subculture method of Oplopanax elaus.[Method] With O.elaus as material,the effect of different explants,mediums and hormones on callus induction was studied.[Result] The results showed that leaf was the best suitable organs for tissue culture.May was the best period to draw materials.MS added to 2.0 mg/L 2,4-D and 1.0 mg/L 6-BA had high induction rate on callus;and the optimal subculture medium was MS＋6-BA 2.0 mg/L＋ NAA 0.2 mg/L.[Conclusion] The best callus induction medium and ubculture medium of O.elaus： MS＋2,4-D 1.0 mg/L＋6-BA 1.0 mg/L and MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L.