太湖猪(二花脸)ESR·FSHβ和GPX5基因遗传多态性研究

Polymorphisms of ESR,FSHβ,GPX5 Gene in Taihu Pigs

[目的]检测太湖猪核心保种群体中雌激素受体基因(ESR)、促卵泡素β亚基基因(FSHβ)和谷胱甘肽过氧化物5基因(GPX5)的多态性。[方法]利用PCR和PCR-RFLP方法对太湖猪核心保种群体中ESR、FSHβ、GPX5基因进行多态性检测。[结果]ESR和GPX5基因在太湖猪群体中均检测到AA、AB和BB 3种基因型,FSHβ亚基基因只检测到BB和AB 2种基因型。ESR基因以BB基因型为优势基因型,等位基因B为优势基因。FSHβ基因中等位基因B的频率远大于等位基因A。GPX5基因的基因型频率呈AB>BB>AA趋势。ESR和FSHβ基因为低度多态,GPX5基因处于中度多态。[结论]经χ2适合性检验表明,ESR、FSHβ、GPX5基因均处于Hardy-Weinberg平衡状态(P>0.05)。

[Objective] The aim was to detect the polymorphisms of ESR,GPX5 and FSHβ gene in Taihu Pigs.[Method] PCR and PCR-RFLP were used to analyze the polymorphisms of the three genes.[Result] The results showed that AA,AB,BB genotypes were detected in both Taihu porcine ESR and GPX5 gene,while FSHβ gene was only detected BB and AB genotypes.BB genotype and allele B were predominant of ESR gene.The frequency of allele B was much higher gene than A in FSHβ gene.The genotype frequencies of GPX5 gene had a tendency of AB BB AA.Statistics analysis showed that ESR and FSHβ gene were in low polymorphism,while GPX5 gene was at a moderate polymorphism.[Conclusion] According to the calculated chi-square value,it verified that three genes were all consistent with the Hardy-Weinberg equilibrium（P0.05）