DNA条形码技术在北京百花山地区夜蛾科物种鉴定中的应用

Species identification of Noctuidae moths(Insecta:Lepidoptera) from Baihuashan,Beijing,China with DNA barcoding

为了探讨DNA条形码技术在夜蛾物种鉴定中的可行性,本研究利用条形码通用引物扩增了北京百花山地区43种夜蛾75个样本的线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome c oxidase subunitI,COI)基因序列,以Kimura双参数模型进行种内种间遗传距离分析、使用邻接法(neighbor-joining,NJ)和最大简约法(maximumparsimony,MP)分别构建系统发育树,并利用分子序列差异阈值对样本进行分子可操作分类单元(molecular defined operational taxonomic units,MOTU)划分。结果表明:所有夜蛾种类通过系统发育树可以成功区分;种内平均遗传距离(0.03%)远远小于种间平均遗传距离(11.29%);采用较为保守的1%的序列差异阈值将75个夜蛾样本分为42个MOTU,正确率为95%,除了MOTU04包含2个物种外,剩余41个MOTU与形态种呈现一一对应的关系。结果显示,基于COI基因的DNA条形码对于本研究中所涉及的夜蛾具有较好的区分,可以作为一种有效的工具在夜蛾科昆虫物种鉴定中进行应用。

To explore the feasibility of DNA barcoding in the identification of Noctuidae moths, the COI genes of 75 samples belonging to 43 species from Noctuidae in Baihuashan, Beijing, were amplified using universal barcoding primers. The intraspecies and interspecies genetic distances were calculated using the Kimura-2-parameter model, the phyIogenetic trees were reconstructed by neighbor-joining （N]） and maximum parsimony （MP） method, and the-identification of MOTU was performed using sequence divergence threshold technique. The results showed that all of Noctuidae species were successfully distinguished by the phylogenetic tree. The average intraspecific genetic distance （0.02%） was much less than the average interspecific genetic distance （11.28% ）. Seventy-five Noctuidae samples were divided into 42 MOTUs based on 1% sequence divergence threshold and the accuracy rate was 95%. Most of MOTUs （41/42） were classified into their corresponding morphospecies with only one exception （MOTU04）. The study indicates that the species from Noctuidae family can be well distinguished with the commonly used COI barcodes, which are potentially well utilized in moth species identification.