贵州省黑斑蛙体内裂头蚴扫描电镜观察

Scanning Electron Microscope Observation of Plerocercoid from Rana nigromaculata in Guizhou Province

自野生黑斑蛙体内检获的裂头蚴,选择5条完整、活动度好的虫体,用戊二醛-锇酸双重固定,经乙醇梯度脱水、真空冷冻干燥、喷金后,用扫描电镜观察并拍照。结果发现,虫体前后两端略膨大,不分节,但表面有不规则环形皱褶;体前端无吸槽,顶端中央有一横向凹陷,凹陷周围体壁呈唇状突起;体后端形态类似前端,但中央为一裂隙,裂隙较前端的凹陷浅、窄;体表的质膜内陷形成大小不等的凹窝和沟纹;整个虫体体表密布尖棘状长短不一的微毛。

Five integral and active plerocercoids collected from naturally infected frogs were fixed by glutaraldehyde？-osmic acid, dehydrated in graded series of ethanol, dried via vacuum freeze and coated with carbon gold. They were then observed by scanning electron microscopy. The results showed that the anterior and posterior ends of the plerocercoids were slightly swell and without segmentation. Annular furrows ran irregularly along the body surface. The anterior end was drawn inside and had a deep dorsoventral hollow, around which, the plasma membrane displayed a lip bulge. The posterior end was similar to the anterior one in the shape, but a fissure in its central part was comparatively narrower and shallower than the hollow in the anterior end. There were thousands of pits and grooves on the body surface, unequal in size. The whole body surface of the plerocercoid was densely covered by sharp spine-like microtriches.