种蛋中禽白血病病毒P27抗原检出率与鸡群禽白血病发病率的相关性研究

Detection of Antigen P27 in Eggs and Its Correlation with the Avian Leukosis Virus Infection in Different Breeder or Commercial Chickens

本研究旨在观察种鸡群所产种蛋中禽白血病病毒P27抗原检出率与种鸡群发病状况及其下一代鸡群禽白血病发病率的相关性,为鸡群净化和禽白血病预警提供参考依据。对国内海兰褐蛋鸡等品系不同祖代及父母代共7个种鸡场所产种蛋的P27抗原进行了检测,对部分种鸡场的ALV-A/B和ALV-J抗体进行了检测和病毒的分离鉴定,对各种鸡群孵化后下一代鸡群的禽白血病发病状况和客户投诉情况进行了追踪调查。7个种鸡场所产种蛋中P27抗原检出率为0%～12%,P27抗原检出率与种鸡群的发病状况及其子代鸡群的发病状况和客户投诉情况密切相关,即种蛋中P27抗原检出率越高,种鸡群发病情况越严重,下游客户鸡群发病率和投诉率越高。种鸡群的种蛋P27检出率与种鸡群及其孵化后鸡群的禽白血病感染状况具有较好的吻合性,可以作为开展ALV的流行病学调查和禽白血病感染风险评估的重要指标。

The objective of this study was to investigate the positive rate for antigen P27 of Avian leukosis virus in eggs from different breeder groups and its correlation with the incidence of avian leukemia both in the breeder chickens and its next generation. It is hoped that this study will be helpful for the breeders to estimate the risk of avian leukosis virus infection. Eggs or sera samples from 7 domestic breeder groups from Tianjin, Shandong, Hubei, Hebei province were collected and detected for antigen P27 of ALV and antibodies to ALV-A/B and ALV-J respectively. Some samples with tumors were isolated for exogenous ALV. The tumor report and complaint for the next generation of each breeder were tracted and analyzed. The positive rate of antigen P27 in eggs of different breeders is quite different and the positive rate range from 0 to 12%, positive rate for antigen P27 in eggs closely related to the incidence of ALV infection both for the breeders and its next generation. That is, the higher it is, the more serious for ALV infection and the more complaints from customers. The positive rate for antigen P27 in eggs of breeders conform well to virus isolation and tumor incidence and can be used as an important index to estimate the risk of ALV infection.