OSAHS患者腭帆张肌失神经现象研究

Denervation change of tensor veli palati in obstructive sleep apnea hypopnea syndrome

目的:观察OSAHS患者腭帆张肌中神经细胞黏附分子(NCAM)mRNA及蛋白的表达水平,探讨OSAHS患者腭帆扩张肌失神经变化的情况。方法:选取30例OSAHS患者为实验组,10例排除OSAHS的慢性扁桃体炎患者为对照组。采用实时荧光定量RT-PCR和Western blot方法检测患者腭帆张肌中NCAM mRNA及蛋白的表达水平。结果:①实验组NCAM mRNA及蛋白的表达水平均明显高于对照组,差异有统计学意义(均P<0.05);②实验组NCAM蛋白的表达水平与AHI呈正相关(r=0.803,P<0.01)。结论:OSAHS患者腭帆张肌肌纤维存在失神经变化现象,且随着病情加重,失神经的程度也加重。

To explore the denervation change of Tensor Veli Palati in patients with OSAHS by de terming the mRNA and protein expression of NCAM. Method： The OSAHS group was consisted of 30 OSAHS patients and the normal control group was consisted of 10 chronic tonsillitis patients without OSAHS. Real-time quantitative RT-PCR and Western blot methods were used to determine the NCAM expression in specimens. Resuit：①The mRNA and protein expression level of NCAM in the OSAHS group increased significantly compared with that in control group（P〈0.05）. ②There was positive correlation between AHI and the protein expressio nlevel of NCAM in the OSAHS group（r=0. 803 ,P〈0.01）. Conclusion：These results indicate that the denervation change of tensor veli palati appear in OSAHS patients,and the severity of OSAHS are relevant with the degree of denervation.