热休克蛋白70对大鼠牙髓细胞高温损伤的影响

Effect of Heat Shock Protein 70 on Severity Hyperthermia Induced Injury to Rat Pulp Cells

目的:研究预热处理诱导的热休克蛋白70(HSP70)对受高温损伤的大鼠牙髓细胞的影响。方法:将体外培养的大鼠牙髓细胞随机分为4组,分别为正常对照组(C组):37℃常规培养18h;预热处理组(pH组):37℃常规培养9h后42℃培养1h,再复温培养8h;高温损伤组(SH组):37℃常规培养9h后46℃培养1h,再复温培养8h;预热处理+高温损伤组(pH+SH组):42℃培养1h后恢复常规培养8h,再置于46℃培养1h后恢复常规培养8h。以Western Blot检测细胞内HSP70的表达变化;以四唑盐(MTT)比色法、吖啶橙/溴化乙啶(AO/EB)双染法、细胞形态学观察评价细胞存活能力。结果:Western Blot显示,与C组相比较PH组HSP70表达上调;MTT比色法、AO/EB双染法、细胞形态学观察均显示pH+SH组较SH组的细胞存活能力强。结论:预热处理可诱导大鼠牙髓细胞中的HSP70表达上调,减轻高温对牙髓细胞的损伤。

Objective： To investigate the effects of heat shock protein 70（HSP70） induced by preheating treatment on lethal heat-induced injury to rat pulp cells.Methods： Pulp cells were randomly divided into four groups as following： I control（C）group;II preheating（PH） group was subjected to sublethal heat（42℃×1h）;III severity hyperthermia（SH） group was subjected to lethal heat（46℃×1h）;IV preheating ＋ severity hyperthermia（pH＋SH） group was treated with sublethal heat（42℃×1h） 8h before subjected to lethal heat（46℃×1h）.The expression of HSP70 was detected by Western Blot;the cell viability was measured by MTT,AO/EB and cytomorphology.Results： As Western Blot revealed： compared with C group,the expression of HSP70 in PH group was upregulated;as concluded respectively by MTT,AO/EB and cytomorphology,the cell in PH＋SH group was of great viability.Conclusion： Preheating treatment can upregulate the expression of HSP70 in rat pulp cells,thus protecting the cells from lethalheat injury.