慢性牙周炎患者牙龈组织内诱导型一氧化氮合酶表达的研究

The Expression of Inducible Nitric Oxide Synthase in Gingival Tissues of Chronic Periodontitis Patients

目的:研究牙周健康者和慢性牙周炎患者牙龈组织中诱导型一氧化氮合酶的表达强度,探讨一氧化氮在牙周病发病过程中的作用。方法:选择牙周健康组、慢性牙周炎活动期组,慢性牙周炎静止期组各20例,采取免疫组织化学的方法染色,光镜下观察牙龈组织内诱导型一氧化氮合酶的表达强度。结果:慢性牙周炎时牙龈组织中诱导型一氧化氮合酶主要在鳞状上皮和间质组织的细胞胞浆中阳性表达,正常组表达强度弱于慢性牙周炎静止期组和活动期组,慢性牙周炎静止期组表达强度弱于慢性牙周炎活动期组。结论:一氧化氮参与了慢性牙周炎的发生和发展过程,牙龈组织中诱导型一氧化氮合酶的表达强度与慢性牙周炎的炎症程度密切相关。

Objective： To evaluate the expression of inducible nitric oxide synthase（iNOS） in gingival tissues of periodontally healthy individuals and chronic periodontitis patients;and to study the possible role of Nitric Oxide（NO） in the initiation and progression of periodontal disease.Methods： Twenty patients with active chronic periodontitis,20 patients with inactive chronic periodontitis,and 20 periodontally healthy subjects were recruited in this study.Immunohistochemistry was performed to evaluate iNOS expression in gingival tissues under the microscope.Results： The expression of iNOS was mainly observed in epithelial cells and inflammatory cells in connective tissue.The expression level in control group was lower than that in chronic periodontitis groups,which include active and inactive groups.Furthermore,the expression in inactive chronic periodontitis group was lower than that in active group.Conclusion： NO plays an important role in the initiation and progression of chronic periodontal disease.The iNOS expression in gingival tissue closely related with the degree of inflammation.