肝癌病例中XRCC1基因多态性与环境因素的交互作用分析

Interactions between XRCC1 gene polymorphism and specific environmental factors in HCC cases

目的:研究某些环境因素与X射线修复交叉互补组1(XRCC1)基因多态性间交互作用对肝细胞癌(HCC)发生危险性的影响。方法:采用单纯病例研究方法,调查500例HCC患者所接触的相关环境因素,用荧光实时定量PCR方法检测XRCC1-280、XRCC1-194和XRCC1-399单核苷酸多态性。结果:XRCC1-280与乙肝e抗体对HCC的发生有正相乘交互作用(P=0.047,OR=1.809,95%CI:1.007～3.249);XRCC1-194与饮酒对HCC的发生有正相乘交互作用(P=0.041,OR=1.496,95%CI:1.016～2.204);XRCC1-194与乙肝表面抗体阳性对HCC的发生有负相乘交互作用(校正后,P=0.045,OR=1.737,95%CI:1.013～2.979)。未发现XRCC1-399基因多态性与相关环境因素之间存在交互作用。结论:携带XRCC1-194突变等位基因与饮酒、乙肝表面抗体阳性对HCC可能存在交互作用;携带XRCC1-280突变等位基因与乙肝e抗体对HCC可能存在交互作用。

OBJECTIVE： To explore the interactions between environmental exposure factors and genetic polymorphism in XRCC1 in HCC. METHODS： The research was designed by a case-only study based on hospitals. Studied cases were the 500 new HCC cases and their environmental exposed data were collected. TaqMan MGB Real-time quantitative polymerase chain reaction （RT-PCR） was used to detect single nucleotide polymorphism of XRCCl-194, XRCC1-280 and XRCC1-399. RESULTS： XRCC1-280 and Anti-Hbe had a positive multiplieative interaction on HCC occurrence （P= 0. 047, OR= 1. 809, 95 V00CI： 1. 007--3. 249）. XRCC1-194 and alcohol consumption had multiplicative interaction on HCC occurrence（P=0. 041, OR= 1. 496, 95%CI： 1. 016--2. 204）. XRCCl-194 and HBsAb had a negative multiplicative inter- action on HCC occurrence（P= 0. 045, OR： 1. 737, 95 % CI.. 1. 013-- 2. 979）. CONCLUSIONS： Carrying the XRCCl-194 mutant allele and drinking alcohol may have an interaction in HCC; carrying the XRCCl-194 mutant allele and HbsAb tend to an interaction related with HCC. Carrying the XRCC1-280 mutant allele and HbeAb are also likely to have an interaction in HCC.