利用甘蔗糖蜜酒精发酵液生产腐植酸的菌种鉴定及发酵条件研究

Identification of Microorganism Producing Humic Acids with Sugarcane Molasses Alcohol Waste Water and Optimum the Fermentation Conditions

从土壤中筛选出一株适合用甘蔗糖蜜酒精发酵液生产腐植酸的菌株H812。单因素实验和正交实验结果表明,该菌株培养的最适酒精发酵液浓度为16°Bx,最适培养条件为:时间8d、温度34℃、摇床转速200r/min、初始pH7.0、接种量12%和装液量50ml/250ml,其中温度对发酵产品影响显著。在优化的条件下,腐植酸产量为38.12 g/L,较优化前提高了148.34%。对H812菌株进行形态特征分析以及ITS序列分析,推测该菌株为曲霉属真菌。

The H812 strain was screened from sludge,it could make use of sugarcane molasses alcohol waste water to product humic acid,and it was preliminarily identified as Aspergillus sp by its culture characteristics,morphological features and ITS sequence analysis.The results of the monofactorial researchs and orthogonal experiments showed that 16°Bx was the optimal Bx of molasses alcohol waste water.The optimal fermentation conditions were： 8days,34℃,200r/min,pH7.0,inoculum size 12%,liquid volume 50ml/250ml,and the fermentation temperature had significant influence on the production of humic acid.At optimized fermentation conditions,HA content fermented with H812 strain was 38.12 g/L,and increased by 148.34% than before optimization.