摘要
[Objective] On the basis of previous researches, this experiment aimed to analyze the effect of K* and the interaction of K+ , salinity ( s), Ca2+ , Mg2+ on growth and adenosine triphosphatase (ATPase), which was closely related to growth, of Litopenaeus vannamei. [Method] 5 -week Litopenaeus vannamei breeding experiment was performed according to L16(215) orthogonal experiment design. Then the effect of K+ and the interaction of K+ , salinity, Ca2+ , Mg2+ in water on survival, growth and ATP enzyme activity of Litopenaeus vannamei. [Result] K+ had signifi- cant effect on survival rate, length and weight gain, ATP enzyme activity in Litopenaeus vannarnei. The highest survival rate was at K" of 150 rng/L. The fastest growth rate was at 50 mg/L, as well as the activities of Na* - K+ -ATPase, Mg2+ -ATPase, Ca+ -ATPase. The interaction of salini- ty, Ca2+ , Mg2+ , K + in water also had significant effect on survival rate, length and weight gain, ATP enzyme activity in Litopenaeus vannamei. [Conclusion] Among the horizontal combinations of factors in the experiment, the optimal combination for Litopenaeus vannamei survival rate was salinity of 15, K+ of 150 mg/L, Ca2+ of 100 mg/L, Mg2+ of 300 mg/L, the optimal combination for growth rate was salinity of 15, K+ of 50 mg/L, Ca2+ 100 mg/L, Mg2+ 100 mg/L, the optimal combination for Na+ -K* -ATPase, Mg2+ -ATPase, Ca2+ -ATPase activity in Litopenaeus van- namei was salinity of 5, K+ of 50 mg/L, Ca2+ of 100 mg/L, Mg2+ of 100 mg/L.
[Objective] On the basis of previous researches, this experiment aimed to analyze the effect of K* and the interaction of K+ , salinity ( s), Ca2+ , Mg2+ on growth and adenosine triphosphatase (ATPase), which was closely related to growth, of Litopenaeus vannamei. [Method] 5 -week Litopenaeus vannamei breeding experiment was performed according to L16(215) orthogonal experiment design. Then the effect of K+ and the interaction of K+ , salinity, Ca2+ , Mg2+ in water on survival, growth and ATP enzyme activity of Litopenaeus vannamei. [Result] K+ had signifi- cant effect on survival rate, length and weight gain, ATP enzyme activity in Litopenaeus vannarnei. The highest survival rate was at K" of 150 rng/L. The fastest growth rate was at 50 mg/L, as well as the activities of Na* - K+ -ATPase, Mg2+ -ATPase, Ca+ -ATPase. The interaction of salini- ty, Ca2+ , Mg2+ , K + in water also had significant effect on survival rate, length and weight gain, ATP enzyme activity in Litopenaeus vannamei. [Conclusion] Among the horizontal combinations of factors in the experiment, the optimal combination for Litopenaeus vannamei survival rate was salinity of 15, K+ of 150 mg/L, Ca2+ of 100 mg/L, Mg2+ of 300 mg/L, the optimal combination for growth rate was salinity of 15, K+ of 50 mg/L, Ca2+ 100 mg/L, Mg2+ 100 mg/L, the optimal combination for Na+ -K* -ATPase, Mg2+ -ATPase, Ca2+ -ATPase activity in Litopenaeus van- namei was salinity of 5, K+ of 50 mg/L, Ca2+ of 100 mg/L, Mg2+ of 100 mg/L.
基金
Key Projects of Science and Technology Promoting Farm in Shanghai City (Shanghai Agricultural Key Project [2010] No.1-6 )
Science and Technology Promoting Projects of Science and Technology Promoting Farm in Shanghai City(Shanghai Agricultural Promoting Project [2008]No.5 -1 )
Leading Academic Discipline Project of Shanghai Education Commission(J50701)