体外冲击波对兔膝骨性关节炎软骨细胞凋亡的影响

Effect of extracorporeal shock waves on chondrocytes apoptosis of rabbit knee osteoarthritis

目的:探讨体外冲击波(extracorporeal shock wave,ESW)对兔膝骨性关节炎(osteoarthritis,OA)软骨细胞凋亡的影响。方法:18只新西兰兔,根据是否给予前交叉韧带(ACL)离断和ESW干预分为3组(n=6):空白对照组;ACL组;ESW+ACL组。ESW干预8周后,观察膝关节大体情况,并应用DAPI、TUNEL、Annexin Ⅴ-FITC/PI双标流式细胞术三种方法检测对软骨细胞凋亡的影响。结果:DAPI和TUNEL法观察到凋亡软骨细胞主要集中在浅表层、中间层,而Annexin Ⅴ-FITC/PI双标法显示ACL+ESW组有(10.240±0.760)%的软骨细胞凋亡,而ACL组有(17.465±0.621)%的软骨细胞凋亡,两组差异具有统计学意义。结论:ESW可抑制兔膝OA软骨细胞凋亡,减轻对关节软骨破坏,可作为治疗OA的新途径,为临床应用ESW治疗OA提供参考。

Objective To study the extracorporeal shock wave（ESW） on the chondrocyte apoptosis of the rabbit knee osteoarthritis.Methods 18 New Zealand rabbits were divided into three groups（n = 6）：the control group;ACL group;the ESW ＋ ACL group.After 8 weeks of ESW intervention,it observed the knee and detect chondrocyte apoptosis by DAPI,TUNEL and Annexin Ⅴ-FITC/PI flow cytometry methods.Results The results of DAPI and TUNEL observed apoptosis of chondrocytes is mainly concentrated in the shallow surface layer and middle layer.Moreover,the chondrocyte apoptosis in ACLs ＋ ESW group and the ACL group was（10.240 ± 0.760%） and（17.465 ± 0.621）%,which the difference was statistically significant.Conclusion ESW can inhibit chondrocyte apoptosis of rabbit knee OA.ESW can reduce the destruction of articular cartilage,which be used as new ways of treatment of OA.It provides a new clinical application to treatment of OA by ESW.