期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

登革热病毒SCID-LO2人鼠嵌合模型的建立与评价 被引量：1

Development and evaluation of Dengue virus infected SCID-LO2 chimeric mouse model
原文传递
导出
摘要 目的构建登革热病毒(DENV)感染的小动物模型,为阐明其致病机制提供实验材料。方法向严重联合免疫缺陷(SCID)小鼠腹腔内接种人正常肝细胞(LO2)构建"人鼠嵌合体"动物模型,进而腹腔注射DENV,通过检查病毒在体内分布和主要器官的组织学改变,对DENV感染的动物模型进行评价。结果 SCID小鼠成功移植LO2,并且嵌合小鼠感染DENV后出现病毒血症及严重的器官损伤等表现,但无后肢麻痹等无关症状。结论成功构建了SCID-LO2人鼠嵌合模型,嵌合小鼠能够支持DENV复制,并表现出部分人类感染DENV的临床症状,该小鼠感染模型为研究DENV的致病机制提供了有价值的实验材料。 Objective To develop a mouse model for exploration of the pathogenesis of Dengue virus （DENV） infection. Methods Human normal hepatic cell line LO2 was transplanted to severe combined immunodeficiency （SCID） mice intraperitoneally to develop a SCID-LO2 chimeric mouse model. Following challenge with intraperitoneally injected DENV, the model was evaluated based on virus distribution and the histological changes of main organs. Results The LO2 cells were successfully engrafted into SCID mice. SCID-LO2 chimeric mice showed viremia and severe organ injury after the infection of DENV, but without hind-leg paralysis. Conclusion SCID-LO2 chimeric mice can maintain the replication of DENV and manifest some clinical symptoms of DENV infection in humans. Therefore, the SCID-LO2 chimeric mouse model is valuable for studying the pathogenic mechanism of DENV infection.
作者 盛子洋 陈艳雷 王娟 吴娜 范东瀛 王湘 高娜 安静
机构地区 首都医科大学基础医学院微生物教研室
出处 《中国媒介生物学及控制杂志》 CAS CSCD 2012年第5期413-416,共4页 Chinese Journal of Vector Biology and Control
基金 国家自然科学基金(30872227)~~
关键词 登革热病毒 动物模型 人正常肝细胞株 Dengue virus Mouse model L02
分类号 R373.33 [医药卫生—病原生物学]
  • 相关文献

参考文献9

  • 1Cassetti MC,Durbin A,Harris E,et al.Report of an NIAID workshop on dengue animal models[J].Vaccine,2010,28(26):4229-4234.
  • 2An J,Kimura-Kuroda J,Hirabayashi Y,et al.Development of a novel mouse model for Dengue virus infection[J].Virology,1999,263(1):70-77.
  • 3盛子洋,王娟,吴江漫,安静,范东瀛.利用甲基纤维素改良的病毒噬斑技术[J].免疫学杂志,2012,28(4):363-365. 被引量：3
  • 4Blaney JE Jr,Johnson DH,Manipon GG,et al.Genetic basis of attenuation of Dengue virus type 4 small plaque mutants with restricted replication in suckling mice and in SCID mice transplanted with human liver cells[J].Virology,2002,300(1):125-139.
  • 5Johnson AJ,Roehrig JT.New mouse model for Dengue virus vaccine testing[J].J Virol,1999,73(1):783-786.
  • 6Shresta S,Sharar KL,Prigozhin DM,et al.Murine model for dengue virus-induced lethal disease with increased vascular permeability[J].J Virol,2006,80(20):10208-10217.
  • 7Huang KJ,Li SY,Chen SC,et al.Manifestation of thrombocytopenia in dengue-2-virus-infected mice[J].J Gen Virol,2000,81(Pt 9):2177-2182.
  • 8Chen L,Ewing D,Subramanian H,et al.A heterologous DNA prime-Venezuelan equine encephalitis virus replicon particle boost dengue vaccine regimen affords complete protection from virus challenge in cynomolgus macaques[J].J Virol,2007,81(21):11634-11639.
  • 9Paes MV,Pinhao AT,Barreto DF,et al.Liver injury and viremia in mice infected with dengue-2 virus[J].Virology,2005,338(2):236-246.

二级参考文献6

共引文献2

同被引文献17

  • 1Gubler DJ. Epidemic dengue/dengue hemorrhagic fever as a public health, social and economic problem in the 21st century [J]. Trends Microbiol, 2002, 10(2) : 100-103.
  • 2An J, Kimura-Kuroda J, Hirabayashi Y, Yasui K. Development of a novel mouse model for dengue virus infection [J]. Virology, 1999, 263(1): 70-77.
  • 3Cassetti MC, Durbin A, Harris E, Rico-Hesse R, Roehrig J, Rothman A, Whitehead S, Natarajan R, Laughlin C. Report of an NIAID workshop on dengue animal models [J]. Vaccine, 2010, 28(26): 4229-4234.
  • 4Blaney JE Jr, Johnson DH, Manipon GG, Firestone CY, Hanson CT, Murphy BR, Whitehead SS. Genetic basis of attenuation of dengue virus type 4 small plaque mutants with restricted replication in suckling mice and in SCID mice transplanted with human liver cells [J]. Virology, 2002, 300(1) : 125-139.
  • 5Huang KJ, Li SY, Chen SC, Liu HS, Lin YS, Yeh TM, Liu CC, Lei HY. Manifestation of thrombocytopenia in dengue-2-virus-infected mice [J]. J Gen Virol, 2000, 81(Pt9) : 2177-2182.
  • 6Johnson AJ, Roehrig JT. New mouse model for dengue virus vaccine testing [J]. J Virol, 1999, 73(1): 783-786.
  • 7Shresta S, Sharar KL, Prigozhin DM, Beatty PR, Harris E. Murine model for dengue virus-induced lethal disease with increased vascular permeability [J]. J Virol, 2006, 80 (20) : 10208-10217.
  • 8Tan GK, Ng JK, Trasti SL, Schul W, Yip G, Alonso S. A non mouse-adapted dengue virus strain as a new model of severe dengue infection in AG129 mice [J].PLoS Negl Trop Dis, 2010, 4(4): e672.
  • 9Chen L, Ewing D, Subramanian H, Block K, Rayner J, Alterson KD, Sedegah M, Hayes C, Porter K, Raviprakash K. A heterologous DNA prime-Venezuelan equine encephalitis virus replicon particle boost dengue vaccine regimen affords complete protection from virus challenge in cynomolgus macaques [J]. J Virol, 2007, 81(21): 11634-11639.
  • 10Paes MV, Pinhao AT, Barreto DF, Costa SM, Oliveira MP, Nogueira AC, Takiya CM, Farias-Filho JC, Schatzmayr HG, Alves AM, Barth OM. Liver injury and viremia in mice infected with dengue-2 virus [J]. Virology, 2005, 338(2): 236-246.

引证文献1

中国媒介生物学及控制杂志
2012年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部