花生连作红壤芽孢杆菌的群落多样性及其生防效果研究

Research on Diversity of Bacullus Species in Peanut Continuous Cropping Red Soils and Bio-preparation Effect of Bacullus

芽孢杆菌是土壤中常见的微生物类群，在土壤生态系统中占据重要地位。本研究发现低丘红壤区花生连作红壤中芽孢杆菌的数量为2．0×10^5～2．3×10^5cfu／g干土。通过构建3个样品的基因文库，共得到9个种类；16SrDNA测序结果表明该地区芽孢杆菌的优势类型有短小芽孢杆菌、枯草芽孢杆菌、高地芽孢杆菌，分别占总克隆的54％、12％和14％。并研究了施用不同微生物制剂对连作花生产量和生育性状的影响，结果显示，施用光合细菌和枯草芽孢杆菌能够在一定程度上提高花生的产量，枯草芽孢杆菌和光合细菌混合施用的增产效果最好，比对照提高了32．14％。

Bacullus spp. are the common microorganisms in soils, occupying an important position in soil ecosystem. Our study found that the total number of culturable Bacullus spp. was 2.0×10^5～2.3×10^5cfu/g dry soil in peanut continuous cropping red soils. 9 OUTs restriction endonuclease types were Obtained in the three gene libraries. B. pumilus, B. subtilis and B. altitudinis, as the three main restrict/on types, accounted for 54%, 12% and 14% respectively of the total 16S rDNA clones. The application of photosynthetic bacteria and bacillus subtilis could increase peanut yield, particularly when they mixed which could increased the yield by 32.14% compared to the control.