摘要
为研究适宜于大白猪精液常温保存的稀释液配方,以精子活率、质膜完整率、顶体完整率和呼吸强度为测定指标,分析4种不同配方的稀释液常温下保存大白猪精液的效果。结果表明,经48h保存后,稀释液Ⅰ和Ⅱ保存的精子活率、质膜完整性和顶体完整率分别达到54%、58%和88%,显著优于其他组(P<0.05)。经稀释液Ⅰ和Ⅱ保存的精子的呼吸强度分别为7.25min和8.25min,显著优于稀释液Ⅲ、Ⅳ和对照组(P<0.05),但稀释液Ⅰ和Ⅱ差异不显著(P>0.05)。因此,稀释液Ⅰ和Ⅱ是大白猪精液常温保存的适宜稀释液。
In order to develop normal temperature semen preservation diluents to store semen at room temperature for a longer period,fresh semen of Yorkshire was harvested and diluted with four different diluents.Sperm motility,plasma membrane integrity and acrosome integrity rate were measured to assess the diluted semen quality after the preservation.The results showed that sperm motility reached 54%,plasma membrane integrity reached 58% and acrosome integrality achieved 88% in both the diluentsⅠandⅡ,which were vastly superior to the other groups(P0.05).Respiration intensity of semen which were stored in the diluentsⅠandⅡwere 7.25 min and 8.25 min,which were significantly better than the other two diluents and the control(P0.05),nevertheless,the difference between diluentsⅠandⅡwas not significant(P0.05).Therefore,diluentsⅠandⅡare suitable for the normal temperature semen preservation.
出处
《西北农业学报》
CAS
CSCD
北大核心
2012年第9期10-14,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
"十二五"国家科技计划(2011BAD19B04-3)
西安市科技创新支撑计划[NC1118(1)]
陕西省优秀博士学位论文资助项目(A213020901)
关键词
常温保存稀释液
精子活率
质膜完整性
顶体完整性
呼吸强度
Normal temperature preservation diluents
Sperm motility
Plasma membrane integrity
Acrosome integrality
Respiration intensity