甘蓝型油菜隐性核不育系ZWAB的遗传差异分析

Genetic Discrepancy Analysis of Genic Male Sterile Line(ZWAB) in Brassica napus L.

利用50对多态性位点丰富的SSR引物对甘蓝型油菜隐性上位互作核不育系ZWAB与21份不同来源的甘蓝型油菜隐性核不育两系材料进行遗传差异分析。50对引物在22份材料间共检测到139个多态性位点,每对引物检测到1～6个多态性位点,平均2.8个,多态性比率为51.3%。聚类分析表明ZWAB与国内来源于S45 AB、117 AB的隐性核不育两系材料间的遗传差异较大,遗传背景来源于国外的甘蓝型油菜,属一种新型的甘蓝型油菜隐性核不育材料。

The genetic discrepancy of ZWAB and 21 Genic Male Sterile Line from different geographical origins in Brassica napus was analyzed using 50 SSR primers with high polymorphisms. A total of 139 polymorphic alleles were detected from 22 materials, and each pair of primer averagely produced 2.8 alleles ranging from 1 to 6. Polymorphic rate was 51.3 %. The clustering analysis by UPGMA revealed that genetic discrepancy were bigger between ZWAB and Genic Male Sterile Line from S 45 AB or 117 AB origins in internal, and genetic background of ZWAB may origin from Brassica napus in overseas. ZWAB belong to a new Genic Male Sterile Line of Brassica napus L.