利用ISSR分子标记选择烟草GDH88株系的研究

The Selection of Tobacco GDH 88 Strains by Using ISSR Molecular Marker

利用ISSR分子标记技术,对烟草(k 326×8611)×(Corker 176×红大)组合的247个双单倍体植株进行了辅助选择。通过研究,筛选到3个多态性较好、扩增稳定的ISSR引物用于双单倍体植株的选择;从247个双单倍体植株中选择到47个具有k 326和红花大金元主要标记片段的单株,再进一步通过大田观察、鉴定和配合力分析,最后获得优良的GDH 88株系。利用ISSR分子标记技术和系统选育方法相结合,可以选育得到优良的烟草品系,有利于烟草育种效率的提高。

taking advantage of ISSR molecular markers, On tobacco （k 326 × 8611 ） ×（Corker 176× red big ） combinations of 247 double haploid plants were assisted selection. Three better polymorphism ISSR primers which Augmentation was stable were Screened to select double haploid plant,47 individual pieces which has k 326 and red flowers auric yuan main mark were selected from 247 double haploid plants. Through Further the field observation,identify and combining ability analysis. Finally obtained excellent strain of GDH 88, Using the combination of ISSR molecular markers and systenmatie breeding method We can get good breeding strain of tobacco, to the benefit of enhancing the efficiency of tobacco breeding.