IMP3在乳腺癌中的表达及其临床病理意义

Expression of IMP3 and its clinicopathological significance in breast cancer

目的探讨IMP3蛋白在乳腺癌组织中的表达及其与临床病理特征的关系。方法采用免疫组织化学Maxvision法检测103例乳腺癌及相应癌旁正常乳腺标本中IMP3蛋白的表达，同时应用茎环逆转录实时定量PCR（qRT-PCR）检测30例乳腺癌及相应癌旁正常乳腺标本中IMP3mRNA的表达，并分析其与临床病理特征的关系。结果乳腺癌病理类型是浸润性导管癌62例、浸润性小叶癌3例、导管内癌伴微小浸润9例、特殊类型乳腺癌29例，全部乳腺癌与癌旁正常乳腺组织之间IMP3表达（t=19．630，P=0．00）差异有统计学意义。浸润性导管癌中IMP3表达与患者年龄、肿瘤大小、淋巴结转移、组织学分级、pTNM分期、ER、PR、HER-2均未见明显相关（P〉0．05）。与癌旁组织比较，IMP3mRNA在乳腺癌组织中表达显著上调，差异有统计学意义（P〈0．05），而与组织学分级、肿瘤大小及淋巴结转移状态未见明显相关（P〉0．05）。结论IMP3表达上调可能与乳腺癌的发生、发展及侵袭转移有关。

Objective To study the expression of insulin-like growth factor II mRNA-binding protein 3 （IMP3） and its clinicopathological significance in breast cancer. Methods The Maxvision immunohistochemistry was used to detect the expression of IMP3 protein in 103 cases of breast cancer and the adjacent normal breast tis- sues. Stem-loop real-time RT-PCR was used to detect the expression level of IMP3 mRNA in 30 cases of breast cancer and the matched non-tumor adjacent tissues. The relationship between the expression of IMP3 and the clin- icopathological features of breast cancer was analyzed. Results 62 cases were infiltrative ductal carcinoma, 3 cases were invasive lobular carcinoma, 9 cases were microinfiltrative intraductal carcinoma, and 29 cases were special type of carcinoma. The difference of IMP3 expression between breast cancer group and normal breast tis- sue group had statistical significance （ t = 19. 630, P =0. 000） by t test. For infihrative ductal carcinoma patients, IMP3 expression had no significant relation with age, tumor size, lymph node metastasis, histological grade, pT- NM stage, ER, PR, or HER-2 （ P 〉 0. 05 ）. IMP3 mRNA expression level of was significantly higher in tumor tis- sues than in the adjacent normal tissues（P 〈0. 05）. No significant association was found between the expression of IMP3 and the histological grade, tumor size, lymph node metastasis （ P 〉 0. 05 ）. Conclusion It is possible that IMP3 plays an important role in the generation, progression, invasion and metastasis of breast cancer.