组培苗蒸腾速率和水分利用率动态检测

Dynamic Detection of Transpiration Rate and Water Using Efficiency for Plantlets

利用图像分析方法,根据质量守恒定律获得组培苗的生长信息和蒸腾失水信息。通过Logistic方程、线性方程和非线性双曲线方程拟合组培苗生物量与培养时间、水分损失与培养时间以及组培苗蒸腾失水量与生物量形成的关系,建立组培苗生长预测模型、组培苗蒸腾失水模型与组培苗水分利用率模型,并由此计算出组培苗蒸腾速率和水分利用率。通过测定不同氮素水平、继代次数差异下的茅苍术组培苗蒸腾速率和水分利用率,发现茅苍术组培苗的蒸腾速率和水分利用率随培养时间的增加而减少,继代次数少、培养基中氮素含量高的组培苗,蒸腾作用强。

The growth information and transpiration information of plantlets in vitro were acquired by image processing and utilization of the law of mass conservation,respectively.The Logistic,linear and nonlinear hyperbolic equations were used for fitting the relationship of biomass of plantlet in vitro and culture time,water loss and culture time,transpiration water loss and biomass of plantlet in vitro.The models of growth prediction,transpiration of water loss and water using efficiency of plantlet were established.The transpiration rate and water using efficiency of plantlet in vitro were calculated.The transpiration rate and water using efficiency of Atractlodes lancea plantlets in vitro at different nitrogen levels in the medium and subculture generation were measured.The transpiration was strong with less subculture generation and higher nitrogen content in culture medium.