FISH检测9号染色体GLP9 p16基因扩增在膀胱癌临床诊断中的意义

Clinical significance of GLP9 p16 gene amplification detected by FISH in the bladder cancer

目的探讨GLP9 p16基因扩增在诊断膀胱癌中的临床意义。方法应用荧光标记的p16基因探针检测35例膀胱癌和10例腺性膀胱炎患者尿液脱落细胞的染色体及基因畸变情况。结果荧光显微镜下细胞核大且不规则者易观察到染色体基因异常,GLP9 p16多倍体细胞数在膀胱癌中的阳性率为85.7%(30/35),显著高于腺性膀胱炎中的阳性率10.0%(1/10)(P<0.05)。在各分期分级中GLP9 p16多倍体细胞数分别为Ta期17.3±14.9,T1期36.5±27.3,T2期61.8±21.9,T2vs.Ta(P<0.05);G1级14.5±12.0,G2级38.1±24.8,G3级73.5±12.7,随着病理分级的增高GLP9 p16多倍体细胞数显著增加(P<0.05)。结论 FISH检测GLP9 p16基因扩增对膀胱癌的临床诊断有辅助价值,同时也可以作为临床监测膀胱癌进展的一个生物学指标。

Objective To investigate the clinical significance of detecting GLP9 p16 gene amplification in the bladder cancer. Methods Fluorescence-labeled p16 gene probe was used to detect the abnormalities of chromosome 9 in the voided urine from 35 cases of bladder cancer and 10 cases of cystitis glandularis. Results Under fluorescence microscopy, large and irregular nuclei are easily observed as chromosome abnormalities. The positive rate of GLP9 p16 polyploid cells in bladder cancer was 85.7% （30/35）, which was significantly higher than that of cystitis glandularis （10%, 1/10） （ P0.05）. In the different stage of bladder cancer, GLP9 p16 polyploid cell number were Ta（17.3±14.9）, T1（36.5±27.3）, T2（61.8±21.9）, respectively, and T2 vs. Ta （P0.05）. Also, G1（14.5±12.0）, G2（38.1±24.8）, G3（73.5±12.7）, respectively. Furthermore, with pathological grade up-regulated, the number of GLP9 p16 polyploid cells also increased significantly （P0.05）. Conclusion GLP9 p16 gene amplification detected by FISH has important auxiliary value to diagnose bladder cancer more accurately, and can be used as a biological marker to monitor the progression of bladder cancer.