粤北地区瑶族人群载脂蛋白B基因多态性与血脂的关系

Relationship between apolipoprotein B gene polymorphisms and lipid levels in Yao population of Yuebei area

目的探讨粤北地区瑶族人群ApoB基因多态性与血脂的关系。方法应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术对粤北地区瑶族高脂血症患者和健康个体各250例的ApoB进行基因分型,并检测其血脂水平。结果 X＋X-、E＋E-基因型个体中TC、LDL-C水平高于X-X-、E＋E＋纯合型（P〈0.05）,X＋X-、E＋E-基因型个体中TG水平明显高于X-X-、E＋E＋纯合型（P〈0.01）;携带X＋等位基因的个体患高脂血症的风险高于携带X-等位基因者（OR=1.957,95%CI：1.214~3.154）;携带E-等位基因的个体患高脂血症的风险高于携带E＋等位基因者（OR=1.466,95%CI：0.889~2.416）。结论瑶族人群ApoB基因XbaⅠ和EcoRⅠ位点基因多态性与血脂水平有关,X＋和E-等位基因可能是血脂增高的遗传危险因素。

Objective To explore the relationship between ApoB gene polymorphisms and lipid levels in Yao population of Yuebei area. Methods ApoB gene polymorphisms of 250 Yao population patients with hyperlipidemia and 250 healthy peoples were determined by PCR-RFLP. The levels of serum lipid were measured. Results X＋X-, E＋E- genotype TC, LDL-C were higher than X-X-, E＋E＋ homozygotes （P0.05）. X＋X-, E＋E- genotype TG was significantly higher than X-X-, E＋E＋ homozygotes（P0.01）, The X＋ carriers had higher risk on suffering from hyperlipidemia than X- carriers（OR=1.957,95% CI：1.214~3.154）; The E- carriers had higher risk on suffering from hyperlipidemia than E＋ carriers（OR=1.466,95% CI：0.889~2.416）. Conclusion ApoB gene Xba Ⅰ and EcoR Ⅰ polymorphisms and serum lipid levels have a certain relationship in Yao population. The X＋ and E- allele may be the genetic risk factors for hyperlipidemia .