大承气汤高效液相色谱指纹图谱及其与组方药味相关性

High Performance Liquid Chromatographic Fingerprints of Dachengqi Decoction and Its Correlation with Composition Drugs

目的:研究和建立大承气汤汤剂HPLC指纹图谱,为该方的物质基础和质量控制提供了科学依据。方法:采用ZORBAX SB-C18色谱柱(4.6mm×250mm,5μm),甲醇-0.1%磷酸水溶液为流动相,梯度洗脱,流速1mL/min,检测波长220nm,柱温25℃。以柚皮苷为参照物,在相同的色谱条件下测定10批大承气汤汤剂。结果:建立了大承气汤汤剂的HPLC指纹图谱,标定了29个共有指纹峰,通过与对照品的保留时间及紫外光谱比较,指认了芸香柚皮苷、新橙皮苷、柚皮苷、橙皮苷、大黄酸、大黄酚、和厚朴酚、厚朴酚的出峰位置,并进行峰位归属,10批样品的相似度在0.981～0.997之间,该HPLC方法的精密度、稳定性、重现性良好。结论:建立的方法准确、可靠,对大承气汤物质基础的研究提供了一定参考。

Objective：The high performance liquid chromatographic fingerprints of Dachengqi Decoction were estab- lished for providing scientific basis in effective substance foundation and quality control of Dachengqi Decoction. Methods ： A ZORBAX SB - C18 column （ 4.6ram × 250mm,5 μm） was employde ; a linear gradient elution with A （ methyl alcohol） and B （ 0.1% H3 PO4 ） was used ; the flow rate was 1 mL/min ; the detection wavelength was set at 220nm ; the column tem- perature was 25℃. By taking as the reference substance, the fingerprints of 10 batches of Dachengqi Decoction were ana- lyzed under the same chromatographic conditions. Results：The high performance liquid chromatographic fingerprints of Dachengqi Decoction were established, twenty -nine common peaks were signed, eight constiuents were identified as na- rirutin, naringin, hesperidin, neohesperidin, rhein, honokiol, magnolol and chrysophanol by comparing the retention times and ultraviolet spectra of the peaks with those of the reference substances;and the peak belonging place was analyzed, their similarity threshold was between 0.981～ 0. 997 ;the HPLC fingerprints of Dachengqi Decoction established with the above method show good precision, stability and＇ repeatability. Conclusion ： The method is stable and reliable, providing a certain reference to substance biasis of Dachengqi Decoction.