高危HPV感染及hTERC基因在宫颈癌发生发展中的相关性

Correlation of High Risk-HPV Infection and the Human Telomerase Gene in Cervical Cancer

目的:探讨高危人乳头瘤病毒及人类染色体端粒酶基因(hTERC)在宫颈癌发生发展中的关系。方法:2010年10月至2011年05月就诊于哈尔滨医科大学附属第一医院妇科患者445例,采用表面等离子体谐振法(SPR)及荧光原位杂交法(FISH)分别检测高危HPV和hTERC的表达情况,对任一结果阳性者行阴道镜下宫颈活检和病理学检查,其中炎症对照组45例,CIN组33例及宫颈癌组6例。结果:单一型HPV29例,多重型26例,最常见为HPV16型合并的二型感染(17例)。在炎症组,CINⅠ组,CINⅡ组、CINⅢ组及宫颈癌组中多重高危HPV比率为17.78%、28.57%、44.44%、50.00%、66.67%,与炎症对照组比较其他组均有统计学意义(P<0.05)。hTERC基因在各组中的阳性表达率分别为2.22%、14.29%、61.11%、75.00%及100%。CIN组及宫颈癌的hTERC基因表达与炎症组比较差异均有统计学意义(P均<0.01),其中CINⅡ及以上各组差异明显。多重HPV感染与hTERC表达呈中等正相关(r=0.462,P<0.01)。结论:随着宫颈病理类型分级升高,HPV感染及hTERC基因表达率升高,hTERC基因在宫颈癌发生发展中起重要作用;高危HPV感染与hTERC基因的异常扩增关系密切,提示二者可能存在因果关系。

Objective： To investigate the relationship of HR-HPV infection and the Human Telomerase Gene（hTERC） in the generation and progression of cervical cancer.Methods： The cervical samples of 445 women visited the First Clinical College of Harbin Medical University from October 2010 to May 2011 underwent the test for HR-HPV with SPR method.hTERC expression was detected by FISH.Then the sample with any positive result underwent colposcopic indicated biopsy and pathological diagnosis.Results： The pathological results showed that 45 of inflammation,33of CIN and 6 Cervical cancer.Among the 445 cases,there were 29 of single HR-HPV infection and 26 of multi-infection,including HPV 16/X double infection（17/26）.The rate of multiple HR-HPV infection was 66.67% in cervical cancer group,28.57% in CINⅠgroup,44.44% in CINⅡgroup,50.00% in CINⅢ group and 30.56% in inflammation group,respectively.The infection rate of any group was higher than that in inflammation group（all P0.05）.The positive amplification rate of hTERC in inflammation group was 2.22%,while it was 14.29% for CINⅠ,61.11% for CINⅡ,75.00% for CINⅢand the cervical 100%for cancer,which showed a significant difference（all P0.01）,evidently in CINⅡand CINⅡ＋.The multiple HR-HPV infection was middle-positive correlated to hTERC expression（r=0.462,P0.01）.Conclusions： As the development of cervical cancer,the HR-HPV Infection and hTERC expression increased and hTERC gene may play an important part in the pathogenesis of cervical cancer.The HR-HPV infection may be a positive-stimulated fact of hTERC gene.