不同浓度帕瑞昔布对结肠癌LoVo细胞增殖及凋亡的影响

Effects of different concentrations of parecoxib on proliferation and apoptosis of colon cancer LoVo cells

目的评价帕瑞昔布对结肠癌LoVo细胞增殖及凋亡的影响。方法将结肠癌LoVo细胞接种于培养板培养24h，采用随机数字表法，将细胞分为对照组（C组）和不同浓度帕瑞昔布处理组（P1-3组），每组6孔。对照组正常培养，P1-3组分别采用10、40、160μmol／L帕瑞昔布处理24h。采用MIT法测定细胞增殖抑制率，平板克隆实验测定细胞克隆形成率，流式细胞仪测定细胞凋亡率，RT-PCR法测定SurvivinmRNA和caspase-3mRNA的表达。结果与C组比较，P1-3组细胞增殖抑制率和凋亡率依次升高，克隆形成率依次降低，SurvivinmRNA表达依次下调，caspase-3mRNA表达依次上调（P〈0．05）。结论帕瑞昔布通过下调Survivin，上调caspase-3表达，抑制结肠癌LoV0细胞增殖，促进LoVo细胞凋亡，且其效应呈浓度依赖性。

Objective To investigate the effects of different concentrations of parecoxib on proliferation and apoptosis of colon cancer LoVo cells. Methods The colon cancer LoVo cells were inoculated in culture plate and cultured for 24 h. The cells were randomly divided into 4 groups （ n = 6 each） ： control group （C group） and different concentrations of parecoxib groups （P1.3 groups）. The cells were incubated with parecoxib 10, 40 and 160 #mol/L for 24 h in P1-3 groups respectively. The rates of proliferation inhibition were measured by MTY assay. The colony formation rates were measured by colony formation assay. The apoptotic rate was determined by flow cytome- try. The Survivin and caspase-3 mRNA expression in the cells was detected by RT-PCR. Results Compared with C group, the rates of proliferation inhibition and apoptotic rate were significantly increased, the colony formation rates were significantly decreased, the expression of Survivin mRNA was down-regulated, and the expression of caspase-3 mRNA was up-regulated in a concentration-dependent manner in P1-3 groups （ P 〈 0.05）. Conclusion Parecoxib can inhibit the proliferation of LoVo cells and induce the apoptosis in LoVo cells in a concentration-de- pendent manner through down-regulating the expression of Survivin mRNA and up-regulating the expression of caspase-3 mRNA.