摘要
目的调查16个Y-STR基因座在广西苗族、瑶族、侗族人群中的遗传多态性,考察其法医学应用价值。方法应用AmpF詛STR誖YfilerTMPCR扩增试剂盒对广西地区253名健康志愿者(67名苗族、99名瑶族和87名侗族)进行Y-STR基因座分型检测,统计16个Y-STR基因座的等位基因分布频率和群体遗传学参数,并对3个民族之间的分布差异进行分析。结果在3个民族中DYS385基因座检到的等位基因数最多,而DYS437基因座检到的等位基因数最少。苗族人群中基因多样性(GD)在0.2619(DYS438)~0.9417(DYS385),瑶族人群中GD在0.317 0(DYS391)~0.955 9(DYS385),侗族人群中GD在0.305 3(DYS391)~0.943 3(DYS385)。DYS391和DYS438基因座在3个民族之间差异无统计学意义(P>0.05)。结论这16个常见Y-STR基因座在广西地区3个主要民族中具有法医学应用价值,可用于检案和基础研究。
Objective To investigate the genetic polymorphisms of 16 Y-STR loci and to evaluate the forensic application in Miao, Yao and Dong nationalities of Guangxi population. Methods Genotypes of Y-STR loci were tested in a total of 253 healthy unrelated individuals (67 Miao people, 99 Yao people, 87 Dong people) using AmpFeSTR YfilerTM PCR amplification kit. Allele frequencies and population genetics parameters of the 16 Y-STR loci were statistically analyzed. The allele frequencies were compared among the three nationalities. Results Most alleles were detected at locus DYS385 while fewest alleles were detected at locus DYS437 among the three nationalities. GD values were ranged from 0.261 9 (DYS438) to 0.9417 (DYS385) for Miao nationality, 0.3170 (DYS391) to 0.9559 (DYS385) for Yao nationality and 0.305 3 (DYS391) to 0.943 3 (DYS385)for Dong nationality, respectively. There were no statistically significant differences detected (/9〉0.05) at loci of DYS391 and DYS438 among the three nationalities. Conclusion The 16 Y-STR loci can be applied to practices and basic research of forensic genetics in the three main nationalities of Guangxi population.
出处
《法医学杂志》
CAS
CSCD
2012年第5期355-358,共4页
Journal of Forensic Medicine
基金
广西医疗卫生重点科研课题资助项目(重200805)
关键词
法医遗传学
多态现象
遗传
Y—STR
苗族
瑶族
侗族
forensic genetics
polymorphism, genetic
Y-STR
Miao nationality
Yao nationality
Dong nationality