抗肿瘤坏死因子单抗对内毒素血症家兔脑组织内部血流分配的影响

Effects of anti-rhTNF monoclinal antibody on the blood flow among and within the brain of endotoxemia rabbits

目的 观测内毒素血症时家兔脑组织血流总量及其分配的改变 ,并探讨这种变化的发生机理。方法 静脉注射内毒素造成动物内毒素血症 ,用放射性生物微球法检测注射中和性抗rhTNF单克隆抗体前后脑组织血流量的变化。结果 (1)内毒素组的左右半侧脑组织平均血流量及脑干、脑桥血流量均显著低于对照组 (P <0 0 1) ;(2 )无关抗体组的左右半侧脑组织平均血流量及脑干、脑桥血流量均显著低于抗体保护组 (P <0 0 1)。结论 严重内毒素血症可致脑血流量减少及脑组织内部血流分配紊乱 ,而抗rhTNF单克隆抗体可予以有效纠正。

Objective To measure tissue blood flow distribition and the effects of anti-rhTNF McAb on the distribution of blood flow among and within the brain of endotoxemia rabbits.Methods Endotoxemia was produced by venous endotoxin injection.Radio-labeled-biomicrosphere technique was adapted to measure blood flow distribution.Results (1)The average blood flow in both sides of brain and pallium in endotoxemia group was significantly lower than those of control group( P <0 01).(2)The average blood flow in both sides of brain and pallium in non-relative McAb group was remarkbly lower than that of anti-rhTNF McAb group.Conclusion This experiment suggests that endotoxemia can result in the reduction of brain blood flow and blood distribution,which can be effectively corrected by anti-rhTNF McAb. [