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复合式悬液芯片系统在病原学检测中的临床应用 被引量：2

Clinical application of multiplex suspension array system in pathogen detection
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摘要 复合式悬液芯片系统(multiplex suspension array system,MSAS)由悬浮芯片和微流体芯片组成,具有高通量精确定量的特点,而且具有较高的准确性和重复性,在多种蛋白质分子的同时精确定量和大规模样品检测方面有着很大的应用优势,其应用领域覆盖感染性疾病的快速诊断、细胞因子、信号通路、肿瘤标志物、流行病学病原体的筛查、蛋白质的相互作用、单核苷酸多态性(SNP)的研究等。就MSAS在病原学检测中的应用作一简要综述。 Multiplex suspension array system （MSAS） is composed of suspension array and microfluidic chip, which demonstrates the features of high-throughput, precise quantification, accuracy, and repeatability. MSAS has great applicable advantages in accurate quantification for large-scale sample detection of various protein molecules, such as rapid diagnosis of infectious diseases, screening of cytokines, identification of signaling pathways, development of tumor markers and epidemiological pathogens. It has been used in the investigation of interaction proteins, single nucleotide polymorphism （SNP） analysis, etc. A briefly summary of the applications of MSAS in oathoen detection has been illustrated in this review.
作者 侯斐 王红 罗成 王立魁
机构地区 首都医科大学附属北京友谊医院 天津科技大学食品工程与生物技术学院
出处 《生命科学》 CSCD 2012年第10期1216-1222,共7页 Chinese Bulletin of Life Sciences
基金 教育部留学回国人员科研启动基金[教外司留(2009)1001号]
关键词 复合式悬液芯片系统 病原学检测 悬浮芯片技术 multiplex suspension array system pathogen detection suspension array
分类号 R446.5 [医药卫生—诊断学]
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参考文献40

  • 1Horan PK, Wheeless LL Jr. Quantitative single cell analysis and sorting. Science, 1977, 198(4313): 149-57.
  • 2杨洋,汤华.液相芯片技术在检验医学和生物医学中的应用[J].中国生物化学与分子生物学报,2007,23(4):256-261. 被引量：32
  • 3Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem, 1998, 44(9): 2057-60.
  • 4Biagini RE, Sammons DL, Smith JP, et al. Comparison of a multiplexed fluorescent covalent microsphere immuno- assay and an enzyme-linked immunosorbent assay for measurement of human immunoglobulin G antibodies to anthrax toxins, Clin Diagn Lab Immunol, 2004, 11(1): 50-5.
  • 5Spiro A, Lowe M, Brown D. A bead-based method for multiplexed identification and quantitation of DNA sequences using flow cytometry. Appl Environ Microbiol, 2000, 66(10): 4258-65.
  • 6Ye F, Li MS, Taylor JD, et al. Fluorescent microsphere-based readout technology for multiplexed human single nucleotide polymorphism analysis and bacterial identifica- tion. Hum Mutat, 2001, 17(4): 305-16.
  • 7Pochechueva T, Jacob F, Goldstein DR, et al. Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies. Glycoconj J, 2011, 28(8-9): 507-17.
  • 8van der Wal F J, Achterberg RP, Boer SM, et al. Bead- based suspension array for simultaneous detection of antibodies against the Rift Valley fever virus nucleocapsid and Gn glycoprotein. J Virol Methods. 2012, 183(2): 99- 105.
  • 9Khan IH, Krishnan VV, Ziman M, et al. A comparison of multiplex suspension array large-panel kits for profiling cytokines and chemokines in rheumatoid arthritis patients. Cytometry B Clin Cytom, 2009, 76(3): 159-68.
  • 10Campbell M, Lie WR, Zhao J, et al. Multiplex analysis of Src family kinase signaling by microbead suspension arrays. Assay Drug Dev Technol, 2010, 8(4): 488-96.

二级参考文献65

  • 1Update: outbreak of severe acute respiratory syndrome-Worldwide, 2003. Morb Mort Wkly Rep[R].2003; 52(12): 241-248.
  • 2American CDCP. Preliminary clinical description of severe acute respiratory syndrome. Morb Mortal Wkly Rep[R]. 2003; 52: 255--256.
  • 3Tsang K W, Ho P L, Ooi G C, et al. h cluster of casesof severe acute respiratory syndrome in Hong Kong.N Engl J Med, 2003; 348(20): 1977--1985.
  • 4Poutanen S M, Low D E, Henry B, et al. Identification of severe acute respiratory syndrome in Canada.N Enql J Med, 2003; 348(20): 1995--2005.
  • 5Lee N, Hui D, Wu A, et al. A major outbreak of severe acute respiratory syndrome in Hong Kong. N Enyl J Med, 2003; 348(20): 1986--1994.
  • 6Peiris J S, Lai S T, Poon L L, et al. Coronavirus as a possible cause of severe acute respiratory syndrome.Lancet, 2003; 361(9366): 1319--1325.
  • 7Ksiazek T GI Erdman D, Goldsmith C, et al, A novel coronavirus associated with severe acute respiratory syndrome. N Enyl J Med, 2003; 348(20): 1953--1966.
  • 8Drosten C, Gunther S, Preiser W, et al, Identification of a novel coronavirus in patients with severe acute respiratory syndrome. N Enyl J Med, 2003; 348(20):1967--1976.
  • 9Greensili J, McNamara P S, Dove W, et al. Human metapneumovirus in severe respiratory syncytial virus bronchiolitis. Emerg Infect Dis, 2003; 9(3): 372--375.
  • 10El Fakhry Y, Ouellette M, Papadopoulou B. A proteomic approach to identify developmentally regulated proteins in Leishmania infantum. Proteomics, 2002, 2(8): 1007-1017.

共引文献694

同被引文献20

  • 1杨洋,汤华.液相芯片技术在检验医学和生物医学中的应用[J].中国生物化学与分子生物学报,2007,23(4):256-261. 被引量：32
  • 2卫辰,侯启明,张庶民,等.以百白破疫苗为基础的联合疫苗研究进展和展望[C].第三届传染病防控基础研究与应用技术论坛论文集,2012:333-346.
  • 3Corbel M J, Das RG, Lei D, et al. WHO Working Group on Revision of the Manual of Laboratory Methods for Testing DTP Vaccines-report of two meetings held on 20-21 July 2006 and 28-30 March 2007, Geneva, Switzerland [J]. Vaccine, 2008, 26(16): 1913-1921.
  • 4Pickering JW, Martins TB, Schroder MC, et al. Comparison of a multiplex flow cytometric assay with enzyme-linked immunos- orbent assay for quantitation of antibodies to tetanus, diphtheria, and Haemophilus i:fluenzae type b [J]. Clin Diag Lab Immunol, 2002, 9(4): 872-876.
  • 5European Pharmacopoeia Commission 2005. European pharma- copoeia 5. 0 [S]. Strasburg, France: European Directorate for the Quality of Medicines, 2005.
  • 6Geurtsen J, Fransen F, Vandebriel RJ, et al. Supplementation of whole-cell pertussis vaccines with lipopolysaccharide analogs : modification of vaccine-induced immune responses [J]. Vaccine, 2008, 26(7): 899-906.
  • 7Pickering JW, Martins TB, Greer RW, eta/. A multiplexed fluorescent mierosphere immunoassay for antibodies to pneumo- eoecal capsular polysaccharides [J]. Am J Clin Pathol, 2002, 117 (4) : 589-596.
  • 8Reder S, Riffelmann M, Becker C, et ol. Measuring immunog- lobulin G antibodies to tetanus toxin, diphtheria toxin, and pertussis toxin with single-antigen enzyme-linked immunosorbent assays and a bead-based multiplex assay [J]. Clin Vaccine Immunol, 2008, 15(5): 744-749.
  • 9van Gageldonk PG, van Schaijk FG, van der Klis FR, et ol. Development and validation of a multiplex immunoassay for the simultaneous determination of serum antibodies to Bordetella pertussis, diphtheria and tetanus [J]. J Immunol Methods, 2008, 335(1-2) : 79-89.
  • 10骆鹏,张庶民.百日咳免疫控制研究进展[J].中国公共卫生,2010,26(5):651-653. 被引量：25

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生命科学
2012年 第10期

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