尿D-氨基酸氧化酶的ELISA检测和临床应用

Enzyme immunoassay of D-amino acid oxidase in urine and its clinical application

目的为探索尿Ｄ－氨基酸测定的临床意义。方法 采用多克隆的ＩｇＧ片段和生物素－链霉亲和素－过氧化物酶系统测定尿Ｄ－氨基酸的含量。检测了２１例正常人和４４例各种肾脏疾病患者尿ＤＡＯ浓度，同时测定尿Ｎ－乙酸－β－Ｄ－氨基葡萄糖苷酶（ＮＡＧ）和α１－微球蛋白（ＡＭＧ）。结果尿ＤＡＯ在各种肾脏疾病患者中均有不同程度的增高，并显示尿ＤＡＯ与ＮＡＧ和ＡＭＧ有高度相关性（ｒ＝０．７２９，Ｐ＜０．０１，ｒ＝０．６４３，Ｐ＜０．０１）。结论提示尿 ＤＡＯ检测可作为临床判断肾功能不良的监测指标之一。

Objective In order to study the clincal value of determination of D-amino acid oxidase in urine. Methods We developed an enzyme immunoassay method for the measurement of urinary concentrations of D-amino acid oxidase (DAO), in which the lgG fraction of a polyclonal antibody and the blotinavidin-peroxidase system were used. The urinary concentration of DAO in 21 healthy subjects and in 44 various renal disorders, the concentration of N-acetyl-beta-D-glucosamindase (NAG) and α1-microglobulin (AMG)in the urine were measured and the histological changes of kidney were prelimillarily investigated. Results The findings show a correlation between urinary DAO and urinary NAG(r=0.729,P<0.01) and AMG (r=0.643,P<0.01). Conclusion DAO was judged to he very useful in monitoring the seventy of renal dysfunction.