细胞因子在淋巴细胞介导胶质瘤细胞凋亡中的作用

The effect of cellular factors on glioma cell apoptosis induced by lymphocyte

目的 细胞因子ＴＮＦ、ＩＦＮ等在淋巴细胞介导胶质瘤细胞调亡方面的作用和特点。方法 采用免疫 组化、分子生物学、ＭＴＴ法及ＰＢＬ对培养的胶质瘤细胞凋亡形成的直接观察方法。结果 正常人ＰＢＬ对Ｕ－ ２５１ＭＧ的杀伤活性明显高于ＴＮＦ或ＩＦＮ的单独作用，ＩＦＮ＋ＰＢＬ组对Ｕ－２５１ＭＧ的杀伤作用较 ＰＢＬ及 ＴＮＦ＋ ＰＢＬ组的杀伤作用凋亡细胞很少而核分裂相细胞多；ＰＢＬ＋ＩＦＮ组出现大量肿瘤细胞凋亡。结论 细胞凋亡调节 失常是慢性增殖性肿瘤形成并发展的主要原因。从免疫活性细胞可以通过促凋亡方式抗肿瘤以来，如何通过调节 免疫系统预防和治疗肿瘤已成为目前研究的热点。认为ＭＴＴ法检测ＰＢＬ介导胶质瘤细胞凋亡有特殊意义。在淋 巴细胞抗肿瘤过程中介导释放ＩＦＮ使肿瘤细胞ＴＮＦＲ及ＦａｓＲ表达增强，同时通过胞浆中的穿孔素，颗粒酶的释 放导致肿瘤细胞凋亡。ＴＮＦ引起的肿瘤杀伤作用是与ＴＮＦＲ相关的ＦａｓＲ介导的。ＦａｓＬ及抗Ｆａｓ抗体与Ｆａｓ集合 后导致细胞凋亡。

s: Objective To investigate the effect of cellular factors on glioma cell apoptosis induced by lymphocyte cultured glioma cells;lymphocyte and cellular factors in first 4 hour. Results The killing activity of normal human PBL to U251MG is much higher than TNF or IFN. IFN plus PBL group is the best one,the effect is better than PBL and PBL plus TNF,difference striking(P＜0.05). Contral group has rare apoptosis cells but tumor cell apoptosis. Conclusion Lymphocyte release IFN which increase the tumor cells TNFR and FasR expretion,at the same time, perform and granulin release from plasma and make tumor apoptosis. TNF induce tumor cell wounded is related to TNF and FasR. FasL and antifasantibody connected with Fas and lead to tumor cell apoptosis. It is very significante that using MTT method to research glioma cell apoptosis induced by PBL.