壮骨关节丸对肾虚型骨性关节炎大鼠脏器系数及总胆红素的影响

Effects of Zhuang Gu Guan Jie pills on Kidney Deficiency Osteoarthritis rat organ coefficient and total bilirubin

目的:观察壮骨关节丸对正常大鼠、骨性关节炎及肾虚型骨性关节炎大鼠脏器系数及血清中总胆红素(TBIL)含量的影响,评估其对于骨性关节炎不同证型大鼠模型的影响。方法:采用去势法制作肾虚证模型,肾虚模型建立4周后,采用切断右侧膝关节内前交叉韧带和内侧部分半月板的方法制做骨性关节炎模型。骨性关节炎模型建立后第2天灌胃不同剂量药物,4周后检测大鼠体重和脏器湿重,计算脏器(心、肝、脾、肺、肾、肾上腺、胸腺)系数并测定血清中TBIL含量。结果:各组动物均无死亡,与正常对照组相比,正常高倍量组大鼠胸腺、肝及肾上腺变化明显;肾虚型骨性关节炎模型大鼠胸腺指数显著上升,肝及肾指数显著降低,而肾虚型骨性关节炎高倍量脏器系数却无异常改变。正常高倍量组及骨性关节炎高倍量组TBIL含量相比正常对照组明显升高。结论:壮骨关节丸口服对不同证型骨性关节炎大鼠主要脏器影响程度不同,尤其对于正常大鼠影响较大,推测壮骨关节丸毒性反应与疾病的证候类型相关。

Objective To observe the impacts of Zhuanggu-guanjie Pill （ZGGJP） on organ coefficients and total bilirubin （TBIL） in normal rats, osteoarthritis rats and osteoarthritis rats with kidney deficient, and to evaluate the pill＇s pharmacological effect on osteoarthritis rats model with different syndrome. Methods： Oophorectomy method was used to build kidney deficient model, 4 weeks later, anterior cruciate ligament and partial medial meniscus of right knee joint were cut to build osteoarthritis model. Drugs were oral administrated in low or high dosages after osteoarthritis operation, and lasted for 4 weeks. The body mass and organs mass were weight their organ coefficients, such as heart, liver, spleen, lung, kidneys, adrenal glands, and thymus gland and so on, were calculated and TBIL concentration in serum were tested also. Results： There was no rats died in the experimental period. Compared with normal control rats, organ coefficients of thymus gland, liver and adrenal glands in normal rats with high dosages of ZGGJP were significantly higher. Whereas in kidney deficient osteoarthritis rats, the thymus gland coefficient was obviously elevated, but the liver and kidney coefficients were significantly decreased. In addition,the TBIL contents in both normal rats and osteoarthritis rats were higher than that in normal control rats. Conclusion： The effects of ZGGJP oral administration in different types of syndromes were different, and were greater in normal rats, this clued the toxicity of ZGGJP was related with different syndrome types of diseases which were treated. These findings suggest that the drugs were applied according to syndrome differentiation so that a better clinical effect was obtained.