头穴丛刺法对急性脑梗死大鼠行为学及凋亡相关基因bcl-2的影响

Effects of Cluster Scalp Acupuncture on Behavior and Apoptosis-related Gene bcl-2 in Rats after Acute Cerebral Infarction

目的探讨头穴丛刺对急性脑梗死大鼠神经行为学及凋亡相关基因bcl-2的影响。方法将72只雄性Wistar大鼠随机分为3组:假手术组(A)、造模组(B)和头穴丛刺组(C),每组各24只。各组再根据脑梗死后不同时间分为6 h、24 h和3 d三个亚组,每个时间点各8只。采用线栓法制备急性脑梗死动物模型,观察不同时间点头穴丛刺法对急性脑梗死大鼠行为学及脑组织凋亡相关基因bcl-2表达的影响。结果脑梗死第3天,C组大鼠神经功能评分明显低于B组(P<0.01);C组大鼠术后不同时间点缺血半暗区bcl-2的表达增多,24 h时达到高峰,与B组比较差异均具有高度显著性差异(P<0.01)。结论头穴丛刺能明显降低急性脑梗死大鼠神经功能评分,促进运动功能恢复;并可促进大鼠缺血半暗区bcl-2的表达,抑制细胞凋亡。

Objective To explore the effects of cluster scalp acupuncture on the behavior and expression of bcl-2 in rats after acute cerebral infarction.Methods 72 male Wistar rats were randomly divided into sham operation group（A）,model group（B） and cluster scalp acupuncture group（C）,24 rats in each group.According to time after cerebral infarction,each group was reassigned to 6 h,24 h and 3 d subgroups,8 rats in each subgroup.The acute cerebral infarction model was established with the middle cerebral artery occlusion.The effects of cluster scalp acupuncture on the behavior and expression of apoptosis-related gene bcl-2 in the rat brain tissues with acute cerebral infarction were tested at different time.Results 3 d after cerebral infarction,the score of neuronal function in group C was significantly lower than that of group B（P〈0.01）.The expression of bcl-2 in brain ischemic penumbra increased in group C at different time and reached its peak at 24 h.Compared with group B,there was a statistical difference（P〈0.01）.Conclusion Cluster scalp acupuncture can significantly reduce the neuronal function score of rats after acute cerebral infarction and then promote the recovery of movement function.The therapy can inhibit the apoptosis by increasing the expression of bcl-2 in penumbra zone of cerebral infarction.