抗SPAG9自身抗体在肿瘤患者外周血中表达分析

Research on expression of anti-SPAG9 autoantibody in peripheral blood of cancer patients

目的通过对各种肿瘤患者血清中精子相关抗原9(SPAG9)自身抗体的检测初步了解肿瘤患者外周血中抗SPAG9自身抗体的表达情况。方法采用重组SPAG9建立ELISA检测肝癌、肺癌以及其他肿瘤患者血清中的抗SPAG9自身抗体水平,以了解SPAG9引起的肿瘤患者体液免疫反应。以健康对照组血清中抗SPAG9自身抗体OD值的均值加2倍SD作为阳性判断标准。结果 86例肿瘤患者血清中抗SPAG9自身抗体总体阳性率为32.6%,肝癌组阳性率为48.1%,肺癌组为16.2%,其他肿瘤组为40.8%。结论 SPAG9自身抗体在各种肿瘤患者外周血中均有不同程度的表达,值得进一步研究。

Objective To discuss the expression of anti-SPAG9 autoantibody in peripheral blood of cancer patients by testing the anti-SPAG9 autoantibody in serum of cancer patients.Methods ELISA built by SPAG9 recombinant protein for the detection the levels of serum SPAG9 antibodies in liver cancer,lung cancer and other cancers patients to monitor the immune response against SPAG9 in cancer patients.The positive criterion was titers above the mean±2 SD of the healthy sera.Results The total positive rate of 86 cancer patients was 32.6%;liver cancer group was about 48.1%;lung group was just 16.2% and others was 40.8%.Conclusion Anti-SPAG9 autoantibody expression in peripheral blood of various cancer patients with different degree which worthiness further study.